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Invitrogen
NAMPT Monoclonal Antibody (362616)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
NAMPT Antibody (MA5-24108) in WB
Western blot was performed using Anti-NAMPT Monoclonal Antibody (362616)(Product # MA5-24108) and a 55 kDa band corresponding to NAMPT was observed across cell lines tested. Increased expression of NAMPT was observed in HUVEC upon IL1-beta and TNF-alpha treatment. Whole cell extracts (30 µg lysate) of HUVEC (Lane 1), HUVEC treated with IL1-beta (10 ng/mL for 18hr) (Lane 2), HUVEC treated with TNF-alpha (10 ng/mL for 18hr) (Lane 3) and U-87 MG (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Reso... View More
Product Details
MA5-24108
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
362616
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PBEF/Visfatin Met1-His491
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608751
Product Specific Information
In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human PBEF is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp666B131; EC 2.4.2.12; MGC117256; NAmPRTase; Nicotinamide phosphoribosyltransferase; PBEF; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; Visfatin
Gene Aliases: 1110035O14Rik; AI314458; AI480535; NAmPRTase; NAMPT; PBEF; PBEF1; VF; VISFATIN
UniProt ID: (Human) P43490, (Mouse) Q99KQ4
Entrez Gene ID: (Human) 10135, (Mouse) 59027
nicotinamide metabolic process
signal transduction
cell-cell signaling
female pregnancy
circadian rhythm
positive regulation of cell proliferation
insulin receptor signaling pathway
NAD biosynthetic process
response to organic cyclic compound
circadian regulation of gene expression
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of nitric-oxide synthase biosynthetic process
adipose tissue development
pyridine nucleotide biosynthetic process
rhythmic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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