Proteintech

NCL Polyclonal Antibody

NCL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

NCL Antibody (10556-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (Product # 10556-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

NCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NCL Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10556-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:200

View 12 publications 12 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Insect, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NCL Fusion Protein Ag0859 (1-482 aa encoded by BC006494)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MVKLAKAGK NQGDPKKMAP PPKEVEEDSE DEEMSEDEED DSSGEEVVIP QKKGKKAAAT SAKKVVVSPT KKVAVATPAK KAAVTPGKKA AATPAKKTVT PAKAVTTPGK KGATPGKALV ATPGKKGAAI PAKGAKNGKN AKKEDSDEEE DDDSEEDEED DEDEDEDEDE IEPAAMKAAA AAPASEDEDD EDDEDDEDGK SKGIAYIEFK TEADAEKTFE EKQGTEIDGR SISLYYTGEK GQNQDYRGGK NSTWSGESKT LVLSNLSYSA TEETLQEVFE KATFIKVPQN QNGKSKGYAF IEFASFEDAK EALNSCNKRE IEGRAIRLEL QGPRGSPNAR SQPSKTLFVK GLSEDTTEET LKESFDGSVR ARIVTDRETG SSKGFGFVDF NSEEDAKAAK EAMEDGEIDG NKVTLDWAKP KGEGGFGGRG GGRGGFGGRG GGRGGRGGFG GRGRGGFGGR GGFRGGRGGG GDHKPQGKKT KFE (1-482 aa encoded by BC006494)

Target Information

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nucleophilic covalent ligand discovery for the cysteine redoxome.

Nature chemical biology

Fu L,Jung Y,Tian C,Ferreira RB,Cheng R,He F,Yang J,Carroll KS

Wed Nov 01 00:00:00 EDT 2023

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing.

Clinical and translational medicine

Chen QW,Cai QQ,Yang Y,Dong S,Liu YY,Chen ZY,Kang CL,Qi B,Dong YW,Wu W,Zhuang LP,Shen YH,Meng ZQ,Wu XZ

Sun Jan 01 00:00:00 EST 2023

Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment.

Nature structural & molecular biology

Li D,Cao R,Li Q,Yang Y,Tang A,Zhang J,Liu Q

Sat Jul 01 00:00:00 EDT 2023

Antioxidant and Immunomodulatory Properties of Partially purified Exopolysaccharide from Lactobacillus Casei Isolated from Chinese Northeast Sauerkraut.

Immunological investigations

Xu X,Qiao Y,Peng Q,Shi B,Dia VP

Sun May 01 00:00:00 EDT 2022

Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth.

Oncogenesis

Gu C,Zhang W,Yang E,Gu C,Zhang Z,Ke J,Wang X,Wu S,Li S,Wu F

Thu Sep 15 00:00:00 EDT 2022

Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth.

Oncogenesis

Gu C,Zhang W,Yang E,Gu C,Zhang Z,Ke J,Wang X,Wu S,Li S,Wu F

Thu Sep 15 00:00:00 EDT 2022

Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth.

Oncogenesis

Gu C,Zhang W,Yang E,Gu C,Zhang Z,Ke J,Wang X,Wu S,Li S,Wu F

Thu Sep 15 00:00:00 EDT 2022

Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth.

Oncogenesis

Gu C,Zhang W,Yang E,Gu C,Zhang Z,Ke J,Wang X,Wu S,Li S,Wu F

Thu Sep 15 00:00:00 EDT 2022

Fast detection, a precise and sensitive diagnostic agent for breast cancer.

Journal of experimental & clinical cancer research : CR

Wu Q,Yuan C,Liu N,Shu J,Wang J,Qian J,Zeng L,Zhang H,Wang X,Mei W

Mon Jun 13 00:00:00 EDT 2022

Human histone pre-mRNA assembles histone or canonical mRNA-processing complexes by overlapping 3'-end sequence elements.

Nucleic acids research

Ielasi FS,Ternifi S,Fontaine E,Iuso D,Couté Y,Palencia A

Mon Nov 28 00:00:00 EST 2022

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells.

Genes & diseases

Tan R,Du W,Liu Y,Cong X,Bai M,Jiang C,Li Z,Tan M,Ma DK,Huang Q,Jiang W,Dang Y

Sun May 01 00:00:00 EDT 2022

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells.

Genes & diseases

Tan R,Du W,Liu Y,Cong X,Bai M,Jiang C,Li Z,Tan M,Ma DK,Huang Q,Jiang W,Dang Y

Sun May 01 00:00:00 EDT 2022

Downregulation of NCL attenuates tumor formation and growth in HeLa cells by targeting the PI3K/AKT pathway.

Cancer medicine

Ying J,Pan R,Tang Z,Zhu J,Ren P,Lou Y,Zhang E,Huang D,Hu P,Li D,Bao Q,Li P

Tue Mar 01 00:00:00 EST 2022

A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes.

Molecular & cellular proteomics : MCP

Song DA,Alber S,Doron-Mandel E,Schmid V,Albus CA,Leitner O,Hamawi H,Oses-Prieto JA,Dezorella N,Burlingame AL,Fainzilber M,Rishal I

Tue Nov 01 00:00:00 EDT 2022

Overexpression of ARHGAP30 suppresses growth of cervical cancer cells by downregulating ribosome biogenesis.

Cancer science

Wu A,Lin L,Li X,Xu Q,Xu W,Zhu X,Teng Y,Yang XM,Ai Z

Mon Nov 01 00:00:00 EDT 2021

Overexpression of ARHGAP30 suppresses growth of cervical cancer cells by downregulating ribosome biogenesis.

Cancer science

Wu A,Lin L,Li X,Xu Q,Xu W,Zhu X,Teng Y,Yang XM,Ai Z

Mon Nov 01 00:00:00 EDT 2021

Self-Assembled DNA Nanostructure as a Carrier for Targeted siRNA Delivery in Glioma Cells.

International journal of nanomedicine

Zhou Y,Yang Q,Wang F,Zhou Z,Xu J,Cheng S,Cheng Y

Fri Mar 26 00:00:00 EDT 2021

Nuclear m⁶A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.

Protein & cell

Chen C,Liu W,Guo J,Liu Y,Liu X,Liu J,Dou X,Le R,Huang Y,Li C,Yang L,Kou X,Zhao Y,Wu Y,Chen J,Wang H,Shen B,Gao Y,Gao S

Tue Jun 01 00:00:00 EDT 2021

The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.

The EMBO journal

Doron-Mandel E,Koppel I,Abraham O,Rishal I,Smith TP,Buchanan CN,Sahoo PK,Kadlec J,Oses-Prieto JA,Kawaguchi R,Alber S,Zahavi EE,Di Matteo P,Di Pizio A,Song DA,Okladnikov N,Gordon D,Ben-Dor S,Haffner-Krausz R,Coppola G,Burlingame AL,Jungwirth P,Twiss JL,Fainzilber M

Mon Oct 18 00:00:00 EDT 2021

Inhibition of Specificity Protein 1 Is Involved in Phloretin-Induced Suppression of Prostate Cancer.

BioMed research international

Kang D,Zuo W,Wu Q,Zhu Q,Liu P

Thu Apr 29 00:00:00 EDT 2021

ERp57‑small interfering RNA silencing can enhance the sensitivity of drug‑resistant human ovarian cancer cells to paclitaxel.

International journal of oncology

Li S,Zhao X,Chang S,Li Y,Guo M,Guan Y

Tue Jan 01 00:00:00 EST 2019

ERp57‑small interfering RNA silencing can enhance the sensitivity of drug‑resistant human ovarian cancer cells to paclitaxel.

International journal of oncology

Li S,Zhao X,Chang S,Li Y,Guo M,Guan Y

Tue Jan 01 00:00:00 EST 2019

The nuclear matrix protein HNRNPU maintains 3D genome architecture globally in mouse hepatocytes.

Genome research

Fan H,Lv P,Huo X,Wu J,Wang Q,Cheng L,Liu Y,Tang QQ,Zhang L,Zhang F,Zheng X,Wu H,Wen B

Thu Feb 01 00:00:00 EST 2018

The nuclear matrix protein HNRNPU maintains 3D genome architecture globally in mouse hepatocytes.

Genome research

Fan H,Lv P,Huo X,Wu J,Wang Q,Cheng L,Liu Y,Tang QQ,Zhang L,Zhang F,Zheng X,Wu H,Wen B

Thu Feb 01 00:00:00 EST 2018

C9ORF72 hexanucleotide repeat exerts toxicity in a stable, inducible motor neuronal cell model, which is rescued by partial depletion of Pten.

Human molecular genetics

Stopford MJ,Higginbottom A,Hautbergue GM,Cooper-Knock J,Mulcahy PJ,De Vos KJ,Renton AE,Pliner H,Calvo A,Chio A,Traynor BJ,Azzouz M,Heath PR,Kirby J,Shaw PJ

Wed Mar 15 00:00:00 EDT 2017

C9ORF72 hexanucleotide repeat exerts toxicity in a stable, inducible motor neuronal cell model, which is rescued by partial depletion of Pten.

Human molecular genetics

Stopford MJ,Higginbottom A,Hautbergue GM,Cooper-Knock J,Mulcahy PJ,De Vos KJ,Renton AE,Pliner H,Calvo A,Chio A,Traynor BJ,Azzouz M,Heath PR,Kirby J,Shaw PJ

Wed Mar 15 00:00:00 EDT 2017

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Human molecular genetics

Bennion Callister J,Ryan S,Sim J,Rollinson S,Pickering-Brown SM

Thu Dec 01 00:00:00 EST 2016

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Human molecular genetics

Bennion Callister J,Ryan S,Sim J,Rollinson S,Pickering-Brown SM

Thu Dec 01 00:00:00 EST 2016

Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.

Acta neuropathologica

Cooper-Knock J,Higginbottom A,Stopford MJ,Highley JR,Ince PG,Wharton SB,Pickering-Brown S,Kirby J,Hautbergue GM,Shaw PJ

Wed Jul 01 00:00:00 EDT 2015

Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.

Acta neuropathologica

Cooper-Knock J,Higginbottom A,Stopford MJ,Highley JR,Ince PG,Wharton SB,Pickering-Brown S,Kirby J,Hautbergue GM,Shaw PJ

Wed Jul 01 00:00:00 EDT 2015

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Human molecular genetics

Tao Z,Wang H,Xia Q,Li K,Li K,Jiang X,Xu G,Wang G,Ying Z

Fri May 01 00:00:00 EDT 2015

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Human molecular genetics

Tao Z,Wang H,Xia Q,Li K,Li K,Jiang X,Xu G,Wang G,Ying Z

Fri May 01 00:00:00 EDT 2015

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Human molecular genetics

Tao Z,Wang H,Xia Q,Li K,Li K,Jiang X,Xu G,Wang G,Ying Z

Fri May 01 00:00:00 EDT 2015

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Human molecular genetics

Tao Z,Wang H,Xia Q,Li K,Li K,Jiang X,Xu G,Wang G,Ying Z

Fri May 01 00:00:00 EDT 2015

Upregulation of miR-497 induces hepatic insulin resistance in E3 rats with HFD-MetS by targeting insulin receptor.

Molecular and cellular endocrinology

Wang X,Wang M,Li H,Lan X,Liu L,Li J,Li Y,Li J,Yi J,Du X,Yan J,Han Y,Zhang F,Liu M,Lu S,Li D

Sun Nov 15 00:00:00 EST 2015

Upregulation of miR-497 induces hepatic insulin resistance in E3 rats with HFD-MetS by targeting insulin receptor.

Molecular and cellular endocrinology

Wang X,Wang M,Li H,Lan X,Liu L,Li J,Li Y,Li J,Yi J,Du X,Yan J,Han Y,Zhang F,Liu M,Lu S,Li D

Sun Nov 15 00:00:00 EST 2015

Upregulation of miR-497 induces hepatic insulin resistance in E3 rats with HFD-MetS by targeting insulin receptor.

Molecular and cellular endocrinology

Wang X,Wang M,Li H,Lan X,Liu L,Li J,Li Y,Li J,Yi J,Du X,Yan J,Han Y,Zhang F,Liu M,Lu S,Li D

Sun Nov 15 00:00:00 EST 2015

Upregulation of miR-497 induces hepatic insulin resistance in E3 rats with HFD-MetS by targeting insulin receptor.

Molecular and cellular endocrinology

Wang X,Wang M,Li H,Lan X,Liu L,Li J,Li Y,Li J,Yi J,Du X,Yan J,Han Y,Zhang F,Liu M,Lu S,Li D

Sun Nov 15 00:00:00 EST 2015

C9orf72 nucleotide repeat structures initiate molecular cascades of disease.

Nature

Haeusler AR,Donnelly CJ,Periz G,Simko EA,Shaw PG,Kim MS,Maragakis NJ,Troncoso JC,Pandey A,Sattler R,Rothstein JD,Wang J

Thu Mar 13 00:00:00 EDT 2014

C9orf72 nucleotide repeat structures initiate molecular cascades of disease.

Nature

Haeusler AR,Donnelly CJ,Periz G,Simko EA,Shaw PG,Kim MS,Maragakis NJ,Troncoso JC,Pandey A,Sattler R,Rothstein JD,Wang J

Thu Mar 13 00:00:00 EDT 2014

Antisense proline-arginine RAN dipeptides linked to C9ORF72-ALS/FTD form toxic nuclear aggregates that initiate in vitro and in vivo neuronal death.

Neuron

Wen X,Tan W,Westergard T,Krishnamurthy K,Markandaiah SS,Shi Y,Lin S,Shneider NA,Monaghan J,Pandey UB,Pasinelli P,Ichida JK,Trotti D

Wed Dec 17 00:00:00 EST 2014

Antisense proline-arginine RAN dipeptides linked to C9ORF72-ALS/FTD form toxic nuclear aggregates that initiate in vitro and in vivo neuronal death.

Neuron

Wen X,Tan W,Westergard T,Krishnamurthy K,Markandaiah SS,Shi Y,Lin S,Shneider NA,Monaghan J,Pandey UB,Pasinelli P,Ichida JK,Trotti D

Wed Dec 17 00:00:00 EST 2014

Phosphorylation and changes in the distribution of nucleolin promote tumor metastasis via the PI3K/Akt pathway in colorectal carcinoma.

FEBS letters