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NCOA6 Antibody (PA5-100633) in IHC (P)
Immunohistochemistry analysis of NCOA6 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with NCOA6 polyclonal antibody (Product # PA5-100633) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-100633
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NCOA6(Accession Q14686), corresponding to amino acid residues S884-D934.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850139
Product Specific Information
Antibody detects endogenous levels of total NCOA6.
Target Information
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating signal cointegrator 2; activating signal cointegrator-2; Amplified in breast cancer protein 3; amplified in breast cancer-3 protein; ASC-2; Cancer-amplified transcriptional coactivator ASC-2; NRC RAP250; Nuclear receptor coactivator 6; Nuclear receptor coactivator RAP250; nuclear receptor-activating protein 250; Nuclear receptor-activating protein, 250 kDa; peroxisome proliferator-activated receptor interacting protein; peroxisome proliferator-activated receptor interacting protein,PRIP; Peroxisome proliferator-activated receptor-interacting protein; PPAR interacting protein PRIP; PPAR-interacting protein; thyroid hormone receptor binding protein; Thyroid hormone receptor-binding protein
Gene Aliases: AIB3; ASC-2; ASC2; KIAA0181; mKIAA0181; NCOA6; Ncoa7; NRC; PRIP; RAP250; TRBP
UniProt ID: (Human) Q14686
Entrez Gene ID: (Human) 23054, (Mouse) 56406, (Rat) 116464
chromatin binding
transcription coactivator activity
protein binding
ligand-dependent nuclear receptor binding
enzyme binding
protein domain specific binding
estrogen receptor binding
ligand-dependent nuclear receptor transcription coactivator activity
glucocorticoid receptor binding
protein homodimerization activity
peroxisome proliferator activated receptor binding
retinoid X receptor binding
thyroid hormone receptor binding
nuclear hormone receptor binding
retinoic acid receptor binding
chromatin/chromatin-binding, or -regulatory protein
ovarian follicle development
DNA replication
DNA repair
DNA recombination
DNA-templated transcription, initiation
transcription initiation from RNA polymerase II promoter
cellular response to DNA damage stimulus
brain development
heart development
response to hormone
myeloid cell differentiation
intracellular estrogen receptor signaling pathway
positive regulation of insulin secretion involved in cellular response to glucose stimulus
glucocorticoid receptor signaling pathway
cellular lipid metabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
labyrinthine layer blood vessel development
positive regulation of peptide secretion
regulation of nucleic acid-templated transcription
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