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NDUFA9 Antibody (PA5-101693) in ICC/IF
Immunocytochemistry analysis of NDUFA9 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NDUFA9 polyclonal antibody (Product # PA5-101693) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101693
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NDUFA9(Accession Q16795), corresponding to amino acid residues C86-T136.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851127
Product Specific Information
Antibody detects endogenous levels of total NDUFA9.
Target Information
NDUFA9 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial) is also named as NDUFS2L, CI-39kD and belongs to the complex I NDUFA9 subunit family. It is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-39kD; complex I 39kDa subunit; Complex I-39kD; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 2-like (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; NADH-ubiquinone oxidoreductase 39 kDa subunit; ndufa9 {ECO:0000312|EMBL:AAH91192.1, ECO:0000312|RGD:1307307}; short chain dehydrogenase/reductase family 22E, member 1; Sperm flagella protein 3
Gene Aliases: 1010001N11Rik; CC6; CI-39k; CI39k; NDUFA9; NDUFS2L; SDR22E1
UniProt ID: (Human) Q16795, (Rat) Q5BK63, (Mouse) Q9DC69
Entrez Gene ID: (Human) 4704, (Rat) 362440, (Mouse) 66108
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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