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NDUFA9 Antibody (PA5-120861) in WB
Western blot analysis of NDUFA9 in mouse heart. Samples were incubated with NDUFA9 Polyclonal antibody (Product # PA5-120861) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-120861
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NDUFA9 (NP_004993.1).
Conjugate
Form
Liquid
Concentration
1.19 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914433
Product Specific Information
Positive test controls include: LO2, BT-474, HL-60, HT-29, Mouse kidney, Mouse heart, Mouse brain, Mouse liver, Rat testis. The target is usually found in the following locations: Mitochondrion matrix.
Immunogen sequence: MAAAAQSRVV RVLSMSRSAI TAIATSVCHG PPCRQLHHAL MPHGKGGRSS VSGIVATVFG ATGFLGRYVV NHLGRMGSQV IIPYRCDKYD IMHLRPMGDL GQLLFLEWDA RDKDSIRRVV QHSNVVINLI GRDWETKNFD FEDVFVKIPQ AIAQLSKEAG VEKFIHVSHL NANIKSSSRY LRNKAVGEKV VRDAFPEAII VKPSDIFGRE DRFLNSFASM HRFGPIPLGS LGWKTVKQPV YVVDVSKGIV NAVKDPDANG KSFAFVGPSR
Target Information
NDUFA9 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial) is also named as NDUFS2L, CI-39kD and belongs to the complex I NDUFA9 subunit family. It is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-39kD; complex I 39kDa subunit; Complex I-39kD; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 2-like (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; NADH-ubiquinone oxidoreductase 39 kDa subunit; ndufa9 {ECO:0000312|EMBL:AAH91192.1, ECO:0000312|RGD:1307307}; short chain dehydrogenase/reductase family 22E, member 1; Sperm flagella protein 3
Gene Aliases: 1010001N11Rik; CC6; CI-39k; CI39k; NDUFA9; NDUFS2L; SDR22E1
UniProt ID: (Human) Q16795, (Rat) Q5BK63, (Mouse) Q9DC69
Entrez Gene ID: (Human) 4704, (Rat) 362440, (Mouse) 66108
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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