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FIGURE: 1 / 4
NDUFB7 Antibody (PA5-103948) in ICC/IF
Immunocytochemistry analysis of NDUFB7 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NDUFB7 polyclonal antibody (Product # PA5-103948) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103948
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NDUFB7(Accession P17568), corresponding to amino acid residues R52-F102.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853280
Product Specific Information
Antibody detects endogenous levels of total NDUFB7.
Target Information
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase. Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell adhesion protein SQM1; CI-B18; complex I B18 subunit; Complex I-B18; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; NADH-ubiquinone oxidoreductase B18 subunit
Gene Aliases: 1110002H15Rik; B18; CI-B18; NDUFB7
UniProt ID: (Human) P17568, (Mouse) Q9CR61
Entrez Gene ID: (Human) 4713, (Mouse) 66916, (Rat) 361385
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