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Invitrogen
NDUFC2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
NDUFC2 Antibody (PA5-116609) in WB
Western blot analysis of NDUFC2 in extracts of various cell lines. Samples were incubated with NDUFC2 Polyclonal antibody (Product # PA5-116609) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-116609
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NDUFC2 (NP_0045401)
Conjugate
Form
Liquid
Concentration
0.84 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901240
Product Specific Information
Positive Samples: 293T, OVCAR3, HeLa, A-549, mouse stomach, mouse brain, mouse heart, rat kidney
Immunogen sequence: MIARRNPEPL RFLPDEARSL PPPKLTDPRL LYIGFLGYCS GLIDNLIRRR PIATAGLHRQ LLYITAFFFA GYYLVKREDY LYAVRDREMF GYMKLHPEDF
Target Information
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14.5b subunit of the NADH-ubiquinone dehydrogenase complex; CI-49kD; CI-B14.5b; CI-B145b; complex 1, mitochondrial respiratory chain, 49-KD subunit; complex I 49kDa subunit; complex I subunit B14.5b; Complex I-49kD; Complex I-B14.5b; Complex I-B145b; HLC-1; Human lung cancer oncogene 1 protein; NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] 1 subunit C2; NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; NADH-coenzyme Q reductase; NADH-ubiquinone oxidoreductase 49 kDa subunit; NADH-ubiquinone oxidoreductase NDUFS2 subunit; NADH-ubiquinone oxidoreductase subunit B14.5b; ndufs2 {ECO:0000312|EMBL:AAH82067.1}
Gene Aliases: 1810004I06Rik; 2010300P09Rik; AL033311; B14.5b; CI-49; CI-B14.5b; G1; HLC-1; HLC1; NADHDH2; NDUFC2; NDUFS2; R75094
UniProt ID: (Human) O95298, (Human) O75306, (Mouse) Q9CQ54, (Mouse) Q91WD5, (Rat) Q641Y2
Entrez Gene ID: (Human) 4718, (Human) 4720, (Mouse) 68197, (Rat) 293130, (Mouse) 226646, (Rat) 289218
NADH dehydrogenase (ubiquinone) activity
NADH dehydrogenase activity
protein binding
electron carrier activity
ubiquitin protein ligase binding
metal ion binding
quinone binding
NAD binding
4 iron, 4 sulfur cluster binding
oxidoreductase activity
oxidoreductase activity, acting on NAD(P)H
iron-sulfur cluster binding
molecular_function
oxidoreductase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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