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NDUFC2 Antibody (MA5-55366) in WB
Western Blot analysis of various lysates using NDUFC2 Recombinant Monoclonal Antibody (Product # MA5-55366) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
MA5-55366
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9W2N4
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 300-434 of human NDUFS2.
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: WDLRKTQPYD VYDQVEFDVP VGSRGDCYDR YLCRVEEMRQ SLRIIAQCLN KMPPGEIKVD DAKVSPPKRA EMKTSMESLI HHFKLYTEGY QVPPGATYTA IEAPKGEFGV YLVSDGSSRP YRCKIKAPGF AHLAG
Target Information
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14.5b subunit of the NADH-ubiquinone dehydrogenase complex; CI-49kD; CI-B14.5b; CI-B145b; complex 1, mitochondrial respiratory chain, 49-KD subunit; complex I 49kDa subunit; complex I subunit B14.5b; Complex I-49kD; Complex I-B14.5b; Complex I-B145b; HLC-1; Human lung cancer oncogene 1 protein; NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] 1 subunit C2; NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; NADH-coenzyme Q reductase; NADH-ubiquinone oxidoreductase 49 kDa subunit; NADH-ubiquinone oxidoreductase NDUFS2 subunit; NADH-ubiquinone oxidoreductase subunit B14.5b; ndufs2 {ECO:0000312|EMBL:AAH82067.1}
Gene Aliases: 1810004I06Rik; 2010300P09Rik; AL033311; B14.5b; CI-49; CI-B14.5b; G1; HLC-1; HLC1; NADHDH2; NDUFC2; NDUFS2; R75094
UniProt ID: (Human) O95298, (Human) O75306, (Mouse) Q9CQ54, (Mouse) Q91WD5, (Rat) Q641Y2
Entrez Gene ID: (Human) 4718, (Human) 4720, (Mouse) 68197, (Rat) 293130, (Mouse) 226646, (Rat) 289218
NADH dehydrogenase (ubiquinone) activity
NADH dehydrogenase activity
protein binding
electron carrier activity
ubiquitin protein ligase binding
metal ion binding
quinone binding
NAD binding
4 iron, 4 sulfur cluster binding
oxidoreductase activity
oxidoreductase activity, acting on NAD(P)H
iron-sulfur cluster binding
molecular_function
oxidoreductase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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