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NDUFS1 Antibody (PA5-28220) in IHC (P)
NDUFS1 Polyclonal Antibody detects NDUFS1 protein at mitochondria on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. NDUFS1 Polyclonal Antibody (Product # PA5-28220) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-28220
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 312 and 642 of Human NDUFS1
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545696
Product Specific Information
Recommended positive controls: HepG2, mouse brain, rat brain.
Predicted reactivity: Mouse (91%), Rat (92%), Xenopus laevis (82%), Chicken (86%), Chimpanzee (99%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CI-75kD; complex I 75kDa subunit; complex I, mitochondrial respiratory chain, 75-kD subunit; Complex I-75kD; MGC26839; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase); NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; NDUFS-1; OTTHUMP00000206345
Gene Aliases: 5830412M15Rik; 9930026A05Rik; CI-75k; CI-75Kd; NDUFS1; PRO1304
UniProt ID: (Human) P28331, (Rat) Q66HF1, (Mouse) Q91VD9
Entrez Gene ID: (Human) 4719, (Rat) 301458, (Mouse) 227197
protein binding
NADH dehydrogenase (ubiquinone) activity
electron carrier activity
metal ion binding
2 iron, 2 sulfur cluster binding
4 iron, 4 sulfur cluster binding
molecular_function
NADH dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on NAD(P)H
iron-sulfur cluster binding
reductase
mitochondrial electron transport, NADH to ubiquinone
apoptotic mitochondrial changes
mitochondrial respiratory chain complex I assembly
cellular respiration
ATP metabolic process
regulation of mitochondrial membrane potential
reactive oxygen species metabolic process
biological_process
ATP synthesis coupled electron transport
transport
oxidation-reduction process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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