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NECAB2 Antibody (PA5-100635) in ICC/IF
Immunocytochemistry analysis of NECAB2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NECAB2 polyclonal antibody (Product # PA5-100635) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100635
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NECAB2(Accession Q7Z6G3), corresponding to amino acid residues H207-E257.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850141
Product Specific Information
Antibody detects endogenous levels of total NECAB2.
Target Information
The protein encoded by this gene is a neuronal calcium-binding protein that binds to and modulates the function of at least two receptors, adenosine A(2A) receptor and metabotropic glutamate receptor type 5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EF hand calcium binding protein 2; EF-hand calcium binding protein 2; EF-hand calcium-binding protein 2; N-terminal EF-hand calcium-binding protein 2; neuronal calcium binding 2; Neuronal calcium-binding protein 2; Stip-2; Synaptotagmin-interacting protein 2
Gene Aliases: EFCBP2; NECAB2
UniProt ID: (Human) Q7Z6G3, (Mouse) Q91ZP9, (Rat) F1LQY6
Entrez Gene ID: (Human) 54550, (Mouse) 117148, (Rat) 170928
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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