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NELL1 Antibody (PA5-100288) in ICC/IF
Immunofluorescent analysis of NELL1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and NELL1 polyclonal antibody (Product # PA5-100288) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100288
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NELL1(Accession Q92832), corresponding to amino acid residues A612-G662.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2849802
Product Specific Information
Antibody detects endogenous levels of total NELL1.
Target Information
This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NEL-like protein 1; Nel-related protein 1; neural epidermal growth factor-like 1; Protein kinase C-binding protein NELL1
Gene Aliases: B230343H07Rik; IDH3GL; l7R6; NELL1; NRP1
UniProt ID: (Human) Q92832, (Rat) Q62919, (Mouse) Q2VWQ2
Entrez Gene ID: (Human) 4745, (Rat) 81733, (Mouse) 338352
nervous system development
regulation of gene expression
cell differentiation
positive regulation of bone mineralization
negative regulation of osteoblast proliferation
positive regulation of osteoblast differentiation
negative regulation of cyclin catabolic process
positive regulation of apoptotic process
regulation of osteoblast differentiation
positive regulation of ossification
protein homotrimerization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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