Invitrogen

NF1 Monoclonal Antibody (McNFn27a)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA1-084

Applications
Tested Dilution
Publications

Western Blot (WB)

5-10 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-

Affinity Purification (AP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

McNFn27a

Immunogen

Synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560294

Product Specific Information

MA1-084 detects neurofibromin in human, rat and mouse cells.

MA1-084 has been successfully used in Western blot, procedures. By Western blot, this antibody detects a ~230-260 kDa protein.

The MA1-084 immunogen is a synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.

Target Information

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686J1293; FLJ21220; neurofibromatosis 1; Neurofibromatosis type 1; Neurofibromatosis-related protein NF-1; Neurofibromin

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Gene Aliases: AW494271; E030030H24Rik; Nf-1; NF1; NFNS; VRNF; WSS

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UniProt ID: (Human) P21359, (Mouse) Q04690, (Rat) P97526

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Entrez Gene ID: (Human) 4763, (Mouse) 18015, (Rat) 24592

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Function(s)
GTPase activator activity protein binding phosphatidylethanolamine binding phosphatidylcholine binding microtubule binding lipid binding syndecan binding GTPase-activating protein
Process(es)
MAPK cascade osteoblast differentiation metanephros development response to hypoxia liver development negative regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation regulation of cell-matrix adhesion negative regulation of cell-matrix adhesion negative regulation of protein kinase activity cell communication Ras protein signal transduction negative regulation of neuroblast proliferation brain development peripheral nervous system development heart development skeletal muscle tissue development visual learning extrinsic apoptotic signaling pathway via death domain receptors regulation of gene expression Schwann cell development phosphatidylinositol 3-kinase signaling negative regulation of angiogenesis spinal cord development amygdala development forebrain astrocyte development neural tube development cerebral cortex development myelination in peripheral nervous system actin cytoskeleton organization extracellular matrix organization collagen fibril organization adrenal gland development negative regulation of cell migration regulation of synaptic transmission, GABAergic cellular response to heat negative regulation of Rac protein signal transduction wound healing negative regulation of transcription factor import into nucleus positive regulation of apoptotic process regulation of GTPase activity negative regulation of MAP kinase activity negative regulation of MAPK cascade pigmentation positive regulation of neuron apoptotic process regulation of blood vessel endothelial cell migration positive regulation of GTPase activity regulation of bone resorption negative regulation of osteoclast differentiation regulation of glial cell differentiation positive regulation of adenylate cyclase activity regulation of angiogenesis negative regulation of Ras protein signal transduction negative regulation of neurotransmitter secretion negative regulation of fibroblast proliferation regulation of long-term neuronal synaptic plasticity sympathetic nervous system development camera-type eye morphogenesis negative regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation smooth muscle tissue development artery morphogenesis forebrain morphogenesis cognition gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission observational learning regulation of long-term synaptic potentiation positive regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of extrinsic apoptotic signaling pathway in absence of ligand signal transduction negative regulation of cell proliferation regulation of cell proliferation regulation of MAPK cascade regulation of neuron differentiation
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