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NFE2L3 Antibody (PA5-90682) in WB
Western blot analysis of NFE2L3 in extracts of various cell lines. Samples were incubated with NFE2L3 Polyclonal antibody (Product # PA5-90682) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-90682
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 330-550 of human NFE2L3 (NP_0042805)
Conjugate
Form
Liquid
Concentration
2.21 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806173
Product Specific Information
Positive Samples: Raji, U-251MG, Mouse testis, Rat brain, Rat thymus, Rat testis; Cellular Location: Nucleus
Immunogen sequence: DPTARTSQSQ EPFLQLNSHT TNPEQTLPGT NLTGFLSPVD NHMRNLTSQD LLYDLDINIF DEINLMSLAT EDNFDPIDVS QLFDEPDSDS GLSLDSSHNN TSVIKSNSSH SVCDEGAIGY CTDHESSSHH DLEGAVGGYY PEPSKLCHLD QSDSDFHGDL TFQHVFHNHT YHLQPTAPES TSEPFPWPGK SQKIRSRYLE DTDRNLSRDE QRAKALHIPF S
Target Information
NFE2L3 activates erythroid-specific, globin gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NF-E2-related factor 3; NFE2-related factor 3; nuclear factor (erythroid-derived 2)-like 3; nuclear factor erythroid 2-like 3; Nuclear factor erythroid 2-related factor 3; nuclear factor, erythroid 2-like 3; Nuclear factor, erythroid derived 2, like 3; nuclear factor-erythroid 2 p45-related factor 3; nuclear factor-erythroid 2-related factor 3
Gene Aliases: NFE2L3; NRF3
UniProt ID: (Human) Q9Y4A8, (Mouse) Q9WTM4
Entrez Gene ID: (Human) 9603, (Mouse) 18025, (Rat) 312331
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
DNA binding
sequence-specific DNA binding
basic leucine zipper transcription factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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