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NIM1 Antibody (PA5-106735) in ICC/IF
Immunocytochemistry analysis of NIM1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NIM1 polyclonal antibody (Product # PA5-106735) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106735
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NIM1K(Accession Q8IY84), corresponding to amino acid residues E346-R396.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854403
Product Specific Information
Antibody detects endogenous levels of total NIM1.
Target Information
NIM1K serine/threonine kinase or NIM1 belongs to CAMK kinase family. Unlike to other AMPK-related kinases, NIM1 cannot be phosphorylated and activated by LKB1. Two somatic mutations (P333S and P411T) were found in lung neuroendocrine carcinoma and lung large cell carcinoma samples. NIM1 gene ontology (GO) annotations include cellular response to glucose starvation; intracellular signal transduction; negative regulation of TOR signaling; protein phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NIM1 serine/threonine-protein kinase; Serine/threonine-protein kinase NIM1
Gene Aliases: E130304F04Rik; NIM1; NIM1K
UniProt ID: (Human) Q8IY84, (Mouse) Q8BHI9
Entrez Gene ID: (Human) 167359, (Mouse) 245269
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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