Invitrogen

NOX2 Monoclonal Antibody (54.1)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

4 References

View all (19) NOX2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

NOX2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

NOX2 Antibody (MA5-18052) in ICC/IF

Immunocytochemistry analysis of NOX2 in paraformaldehyde fixed MCF7 cells. Samples were incubated with NOX2 monoclonal antibody (Product # MA5-18052) using a dilution of 1:100 for 1hr. Followed by Alexa Fluor® 488 secondary antibody at a 1:1,000 dilution, showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

NOX2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

NOX2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot analysis using NOX2 antibody (Product # MA5-18052), shows increase in expression of NOX2 in THP-1 cells upon PMA and LPS treatment. {TM}

Product Details

MA5-18052

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

1 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

54.1

Immunogen

Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539435

Product Specific Information

This antibody is specific for NOX2/gp91phox (aa 382-389, PKIAVDGP). The immunogen was prepared using partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100).

Western blot results show a band at 65 kDa, which matches the predicted molecular weight. For IHC applications, perform heat-mediated antigen retrieval with citrate buffer, pH 6, prior to staining.

Target Information

Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase complex expresses in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. It includes a membrane bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet possess distinct expression patterns. gp91-phox expresses in eosinophils, neutrophils, monocytes, and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, with low expression in the uterus and prostate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The protective effects of propofol against renal ischemia-reperfusion injury are potentiated by norisoboldine treatment via inhibition of oxidative stress pathways.

Journal of biochemical and molecular toxicology

Xing D,Li Q,Lin G,Lin H,Kang W,Zhang M,Ding R,Li N

MA5-18052 was used in Western Blotting to suggest that treatment with NOR potentiated the nephroprotective effects of propofol in rats with I/R-induced renal injury by ameliorating oxidative stress and apoptosis pathway.

Sat Jan 01 00:00:00 EST 2022

The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction.

Biomolecules

Wanschel ACBA,Guizoni DM,Lorza-Gil E,Salerno AG,Paiva AA,Dorighello GG,Davel AP,Balkan W,Hare JM,Oliveira HCF

MA5-18052 was used in Western Blot, Immunocytochemistry to support the notion that CETP expression negatively impacts endothelial cell function, revealing a new mechanism that might contribute to atherosclerosis.

Thu Jan 07 00:00:00 EST 2021

The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction.

Biomolecules

Wanschel ACBA,Guizoni DM,Lorza-Gil E,Salerno AG,Paiva AA,Dorighello GG,Davel AP,Balkan W,Hare JM,Oliveira HCF

Thu Jan 07 00:00:00 EST 2021

The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction.

Biomolecules

Wanschel ACBA,Guizoni DM,Lorza-Gil E,Salerno AG,Paiva AA,Dorighello GG,Davel AP,Balkan W,Hare JM,Oliveira HCF

Thu Jan 07 00:00:00 EST 2021

The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction.

Biomolecules

Wanschel ACBA,Guizoni DM,Lorza-Gil E,Salerno AG,Paiva AA,Dorighello GG,Davel AP,Balkan W,Hare JM,Oliveira HCF

Thu Jan 07 00:00:00 EST 2021

Alisol B 23-Acetate Ameliorates Lipopolysaccharide-Induced Cardiac Dysfunction by Suppressing Toll-Like Receptor 4 (TLR4)/NADPH Oxidase 2 (NOX2) Signaling Pathway.

Medical science monitor : international medical journal of experimental and clinical research

Wang B,Chen L,Dai L,Fang W,Wang H

Published figure using NOX2 monoclonal antibody (Product # MA5-18052) in Western Blot

Sun Nov 10 00:00:00 EST 2019

Bioinformatics

Protein Aliases: CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 beta polypeptide; Cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; GP91 PHOX; gp91-1; gp91-phox; GP911; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; p91PHOX; Superoxide-generating NADPH oxidase heavy chain subunit

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Gene Aliases: AMCBX2; CGD; CYBB; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX

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UniProt ID: (Human) P04839

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Entrez Gene ID: (Human) 1536

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Function(s)

voltage-gated ion channel activity protein binding electron carrier activity superoxide-generating NADPH oxidase activity heme binding metal ion binding protein heterodimerization activity flavin adenine dinucleotide binding oxidase

Process(es)

response to reactive oxygen species antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent superoxide metabolic process ion transport inflammatory response regulation of ion transmembrane transport superoxide anion generation innate immune response cell redox homeostasis respiratory burst vascular endothelial growth factor receptor signaling pathway hydrogen peroxide biosynthetic process oxidation-reduction process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NOX2 Monoclonal Antibody (54.1)

NOX2 Antibody (MA5-18052) in ICC/IF

Product Details

MA5-18052

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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