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FIGURE: 1 / 1
NTAN1 Antibody (PA5-90558) in WB
Western blot analysis of NTAN1 in extracts of various cell lines. Samples were incubated with NTAN1 Polyclonal antibody (Product # PA5-90558) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-90558
Product Specifications
Species Reactivity
Rat,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human NTAN1 (NP_7757451)
Conjugate
Form
Liquid
Concentration
2.89 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806116
Product Specific Information
Immunogen sequence: MPLLVEGRRV RLPQSAGDLV RAHPPLEERA RLLRGQSVQQ VGPQGLLYVQ QRELAVTSPK DGSISILGSD DATTCHIVVL RHTGNGATCL THCDGTDTKA EVPLIMNSIK SFSDHAQCGR LEVHLVGGFS DDRQLSQKLT HQLLSEFDRQ EDDIHLVTLC VTELNDREEN ENHFPVIYGI AVNIKTAEIY RASFQDRGPE EQLRAARTLA GGPMISIYDA ETEQLRIGPY SWTPFPHVDF WLHQDDKQIL ENLSTSPLAE PPHFVEHIRS TLMFLKKHPS PAHTLFSGNK ALLYKKNEDG LWEKISSPGS; Positive Samples: 293T, Raji, HepG2, MCF-7, Mouse spleen, Rat kidney; Cellular Location: Cytoplasm
Target Information
Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: asparagine-specific N-terminal amidase; N-terminal Asn amidase; N-terminal asparagine amidohydrolase; PNAA; PNAD; protein N-terminal Asn amidase; protein N-terminal asparagine amidase; Protein N-terminal asparagine amidohydrolase; Protein NH2-terminal asparagine amidohydrolase; Protein NH2-terminal asparagine deamidase; protein NTN-amidase
Gene Aliases: NTAN1; PNAA; PNAD; RGD1305160
UniProt ID: (Human) Q96AB6, (Mouse) Q64311
Entrez Gene ID: (Human) 123803, (Rat) 360462, (Mouse) 18203
protein-N-terminal asparagine amidohydrolase activity
histone deacetylase activity
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity
hydrolase activity
iprodione amidohydrolase activity
(3,5-dichlorophenylurea)acetate amidohydrolase activity
4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity
didemethylisoproturon amidohydrolase activity
N-isopropylacetanilide amidohydrolase activity
N-cyclohexylformamide amidohydrolase activity
isonicotinic acid hydrazide hydrolase activity
cis-aconitamide amidase activity
gamma-N-formylaminovinylacetate hydrolase activity
N2-acetyl-L-lysine deacetylase activity
O-succinylbenzoate synthase activity
indoleacetamide hydrolase activity
N-acetylcitrulline deacetylase activity
N-acetylgalactosamine-6-phosphate deacetylase activity
diacetylchitobiose deacetylase activity
chitooligosaccharide deacetylase activity
protein modifying enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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