Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
NTF4 Antibody (500-P83BT-25UG) in WB
Western Blot: To detect Human NT-4 by Western Blot analysis NT4 Polyclonal Antibody, Biotin (Product # 500-P83BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human NT-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P83BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 28.1kDa Recombinant Human NT-4
Conjugate
Form
Lyophilized
Purification
Affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2942237
Product Specific Information
AA Sequence of recombinant protein (monomer): GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NT-4. Anti-Human NT-4-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human NT-4 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NT-4 (500-P83) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human NT-4.
Western Blot: To detect Human NT-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human NT-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P83BT-1MG will be provided as 2 x 500 µg
Target Information
This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: neurotrophic factor 4; neurotrophic factor 5; Neurotrophin 4/5; neurotrophin 5 (neurotrophin 4/5); Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4; Neutrophic factor 5; NT-4; NT-5; NT4/NT5
Gene Aliases: GLC10; GLC1O; NT-4; NT-4/5; NT-5; NT4; NT5; NTF4; NTF5
UniProt ID: (Human) P34130
Entrez Gene ID: (Human) 4909
transmembrane receptor protein tyrosine kinase signaling pathway
cell-cell signaling
ganglion mother cell fate determination
long-term memory
sensory organ boundary specification
adult locomotory behavior
epidermis development
mechanoreceptor differentiation
negative regulation of neuron apoptotic process
regulation of neuron differentiation
neuron projection morphogenesis
innervation
taste bud development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support