Proteintech

NXF1 Polyclonal Antibody

NXF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

NXF1 Antibody (10328-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 10328-1-AP (NXF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NXF1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10328-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NXF1 Fusion Protein Ag0413 (45-349 aa encoded by BC004904)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SGIRSSRLE EDDGDVAMSD AQDGPRVRYN PYTTRPNRRG DTWHDRDRIH VTVRRDRAPP ERGGAGTSQD GTSKNWFKIT IPYGRKYDKA WLLSMIQSKC SVPFTPIEFH YENTRAQFFV EDASTASALK AVNYKILDRE NRRISIIINS SAPPHTILNE LKPEQVEQLK LIMSKRYDGS QQALDLKGLR SDPDLVAQNI DVVLNRRSCM AATLRIIEEN IPELLSLNLS NNRLYRLDDM SSIVQKAPNL KILNLSGNEL KSERELDKIK GLKLEELWLD GNSLCDTFRD QSTYISAIRE RFPKLL (45-349 aa encoded by BC004904)

Target Information

The constitutive transport element (CTE) of type D retroviruses serves as a signal of nuclear export for unspliced viral RNAs. TAP (also known as NXF1) mediates the export of CTE-containing simian type D retroviral RNAs through binding directly to the CTE. TAP is associated with a recognized mRNA export pathway and is a member of the multigene family of NXF proteins. NXF proteins belong to an evolutionarily conserved family of proteins, which are characterized by a leucine-rich-repeat domain (LRR) followed by a region known as the Nuclear Transport Factor 2 (NTF2)-like domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Data of RNA-seq transcriptomes of liver, bone, heart, kidney and blood in klotho mice at a pre-symptomatic state and the effect of a traditional Japanese multi-herbal medicine, juzentaihoto.

Data in brief

Okada N,Oshima K,Iwasaki Y,Maruko A,Matsumura K,Iioka E,Vu TD,Fujitsuka N,Nishi A,Sugiyama A,Nishiyama M,Kaneko A,Mizoguchi K,Yamamoto M,Nishimura S

Wed Jun 01 00:00:00 EDT 2022

Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.

PLoS genetics

Chen M,Long Q,Borrie MS,Sun H,Zhang C,Yang H,Shi D,Gartenberg MR,Deng W

Mon Nov 01 00:00:00 EDT 2021

Intron retention as a new pre-symptomatic marker of aging and its recovery to the normal state by a traditional Japanese multi-herbal medicine.

Gene

Okada N,Oshima K,Iwasaki Y,Maruko A,Matsumura K,Iioka E,Vu TD,Fujitsuka N,Nishi A,Sugiyama A,Nishiyama M,Kaneko A,Mizoguchi K,Yamamoto M,Nishimura S

Fri Aug 20 00:00:00 EDT 2021

The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export.

Molecular cell

Chen S,Wang R,Zheng D,Zhang H,Chang X,Wang K,Li W,Fan J,Tian B,Cheng H

Thu Apr 04 00:00:00 EDT 2019

Intronless mRNAs transit through nuclear speckles to gain export competence.

The Journal of cell biology

Wang K,Wang L,Wang J,Chen S,Shi M,Cheng H

Mon Nov 05 00:00:00 EST 2018

Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy.

Nucleic acids research

Stubbs SH,Conrad NK

Thu Jan 01 00:00:00 EST 2015

Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy.

Nucleic acids research

Stubbs SH,Conrad NK

Thu Jan 01 00:00:00 EST 2015

Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.

The Journal of cell biology

Zhang L,Das P,Schmolke M,Manicassamy B,Wang Y,Deng X,Cai L,Tu BP,Forst CV,Roth MG,Levy DE,García-Sastre A,de Brabander J,Phillips MA,Fontoura BM

Mon Feb 06 00:00:00 EST 2012

Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.

The Journal of cell biology

Zhang L,Das P,Schmolke M,Manicassamy B,Wang Y,Deng X,Cai L,Tu BP,Forst CV,Roth MG,Levy DE,García-Sastre A,de Brabander J,Phillips MA,Fontoura BM

Mon Feb 06 00:00:00 EST 2012

Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.

Molecular and cellular biology

Kim M,Bellini M,Ceman S

Thu Jan 01 00:00:00 EST 2009

Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.

Molecular and cellular biology

Kim M,Bellini M,Ceman S

Thu Jan 01 00:00:00 EST 2009

Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.

Molecular and cellular biology

Kim M,Bellini M,Ceman S

Thu Jan 01 00:00:00 EST 2009

Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.

Molecular and cellular biology

Kim M,Bellini M,Ceman S

Thu Jan 01 00:00:00 EST 2009

XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.

Chromosoma

Cohen HR,Panning B

Wed Aug 01 00:00:00 EDT 2007

XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.

Chromosoma

Cohen HR,Panning B

Wed Aug 01 00:00:00 EDT 2007

XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.

Chromosoma

Cohen HR,Panning B

Wed Aug 01 00:00:00 EDT 2007

XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.

Chromosoma

Cohen HR,Panning B

Wed Aug 01 00:00:00 EDT 2007

XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.

Chromosoma

Cohen HR,Panning B

Wed Aug 01 00:00:00 EDT 2007

XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.

Chromosoma

Cohen HR,Panning B

Wed Aug 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Mex 67 homolog; modifier of vibrator 1; mRNA export factor TAP; Nuclear RNA export factor 1; nuclear RNA export factor 1 homolog; tip associated protein; tip associating protein; Tip-associated protein; Tip-associating protein; Vdp

View more View less

Gene Aliases: MEX67; Mvb1; NXF1; TAP

View more View less

UniProt ID: (Human) Q99799, (Mouse) Q99JX7

View more View less

Entrez Gene ID: (Human) 10482, (Mouse) 53319

View more View less

Function(s)

nucleotide binding single-stranded RNA binding mRNA binding nucleocytoplasmic transporter activity protein binding poly(A) RNA binding RNA binding RNA metabolism protein

Process(es)

RNA export from nucleus mRNA export from nucleus viral process poly(A)+ mRNA export from nucleus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

NXF1 Polyclonal Antibody

NXF1 Antibody (10328-1-AP) in ICC/IF

Product Details

10328-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!