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OAT Antibody (PA5-106699) in ICC/IF
Immunocytochemistry analysis of OAT in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with OAT polyclonal antibody (Product # PA5-106699) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106699
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human OAT(Accession P04181), corresponding to amino acid residues T128-W178.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854367
Product Specific Information
Antibody detects endogenous levels of total OAT.
Target Information
OAT is the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gyrate atrophy; ornithine aminotransferase (gyrate atrophy); Ornithine aminotransferase, mitochondrial; Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase; ornithine-delta-aminotransferase; ornithine-oxo-acid aminotransferase; testicular tissue protein Li 130
Gene Aliases: AI194874; GACR; HOGA; OAT; OATASE; OKT; rOAT
UniProt ID: (Human) P04181, (Mouse) P29758, (Rat) P04182
Entrez Gene ID: (Human) 4942, (Mouse) 18242, (Rat) 64313
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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