Invitrogen

OGT Polyclonal Antibody

View all (19) OGT antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-22092

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 280 of O-GlcNAc transferase (Uniprot ID#O15294)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11152015

Product Specific Information

Recommended positive controls: HeLa, mouse brain, PC-12, Rat2.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Pig (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ23071; MGC22921; O linked N-acetylglucosamine transferase; o-glcnac transferase; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase

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Gene Aliases: 1110038P24Rik; 4831420N21Rik; AI115525; HINCUT-1; HRNT1; O-GLCNAC; OGT; Ogtl

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UniProt ID: (Human) O15294, (Mouse) Q8CGY8, (Rat) P56558

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Entrez Gene ID: (Human) 8473, (Mouse) 108155, (Rat) 26295

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Function(s)
protein binding phosphatidylinositol-3,4,5-trisphosphate binding enzyme activator activity transcription factor binding acetylglucosaminyltransferase activity protein N-acetylglucosaminyltransferase activity protein domain specific binding peptide binding histone acetyltransferase activity (H4-K5 specific) histone acetyltransferase activity (H4-K8 specific) histone acetyltransferase activity (H4-K16 specific) monosaccharide binding protein O-GlcNAc transferase activity catalytic activity N-acetyltransferase activity lipid binding transferase activity transferase activity, transferring glycosyl groups protein modifying enzyme
Process(es)
positive regulation of protein phosphorylation glucosamine metabolic process regulation of glycolytic process protein O-linked glycosylation apoptotic process signal transduction response to nutrient negative regulation of peptidyl-threonine phosphorylation positive regulation of granulocyte differentiation forebrain development negative regulation of protein ubiquitination response to insulin cellular response to insulin stimulus circadian regulation of gene expression negative regulation of peptidyl-serine phosphorylation regulation of Rac protein signal transduction positive regulation of catalytic activity histone H4-K5 acetylation histone H4-K8 acetylation histone H4-K16 acetylation positive regulation of cell size positive regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter regulation of insulin receptor signaling pathway phosphatidylinositol-mediated signaling intracellular distribution of mitochondria positive regulation of histone H3-K4 methylation negative regulation of cell death positive regulation of histone H3-K27 methylation protein homotrimerization protein heterotrimerization cellular response to lipopolysaccharide cellular response to retinoic acid cellular response to glucose stimulus histone H3-K4 trimethylation negative regulation of protein targeting to membrane regulation of gluconeogenesis involved in cellular glucose homeostasis cellular response to toxic substance negative regulation of cellular response to hypoxia positive regulation of protein localization to nucleus positive regulation of reactive oxygen species biosynthetic process negative regulation of protein phosphorylation positive regulation of gene expression chromatin modification rhythmic process
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