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P-Glycoprotein Antibody (PA5-120134) in WB
Western blot analysis of P-Glycoprotein in extracts of various cell lines. Samples were incubated with P-Glycoprotein Polyclonal antibody (Product # PA5-120134) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-120134
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 603-692 of human P Glycoprotein (NP_000918.2).
Conjugate
Form
Liquid
Concentration
1.19 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913706
Product Specific Information
Positive test controls include: Mouse liver, Mouse brain. The target is usually found in the following locations: Cell membrane, Multi-pass membrane protein.
Immunogen sequence: DGVIVEKGNH DELMKEKGIY FKLVTMQTAG NEVELENAAD ESKSEIDALE MSSNDSRSSL IRKRSTRRSV RGSQAQDRKL STKEALDESI
Target Information
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette sub-family B member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B, member 1A; ATP-dependent translocase ABCB1; CD243; ecotropic viral integration site 32; MDR1A; multi-drug resistance 3; multidrug resistance protein 1; Multidrug resistance protein 1A; Multidrug resistance protein 1B; Multidrug resistance protein 3; multiple drug resistant 1a; P glycoprotein 1; P glycoprotein 3; P-glycoprotein 1; P-glycoprotein 3; Phospholipid transporter ABCB1
Gene Aliases: Abcb1; Abcb1a; Abcb1b; Abcb4; Evi32; mdr; mdr-3; Mdr1; Mdr1a; Mdr1b; Mdr3; P-gp; Pgp; Pgy-1; Pgy-3; Pgy1; Pgy1-1; Pgy3
UniProt ID: (Mouse) P21447, (Mouse) P06795
Entrez Gene ID: (Mouse) 18671, (Mouse) 18669
G2/M transition of mitotic cell cycle
transport
drug transmembrane transport
hormone transport
drug transport
xenobiotic transport
daunorubicin transport
drug export
transmembrane transport
negative regulation of cell death
establishment of blood-brain barrier
stem cell proliferation
positive regulation of response to drug
establishment of endothelial blood-brain barrier
response to drug
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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