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Invitrogen
P-Glycoprotein Recombinant Rabbit Monoclonal Antibody (SN06-42)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
P-Glycoprotein Antibody (MA5-32282) in ICC/IF
Immunocytochemistry analysis of P-Glycoprotein with NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp), incubated with recombinant monoclonal P-Glycoprotein (Product # MA5-32282) at a dilution of 1:100 (in 1% BSA in PBST overnight at 4°C), followed by... View More
Product Details
MA5-32282
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SN06-42
Immunogen
Recombinant protein within Human P-glycoprotein 1 aa 373-730
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809567
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABC superfamily; P-glycoprotein; ATP-binding cassette sub-family B (MDR/TAP) member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette sub-family B member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B (MDR/TAP), member 1A; ATP-binding cassette, subfamily B, member 1A; ATP-binding cassette, subfamily B, member 1B; ATP-dependent translocase ABCB1; CD243; colchicin sensitivity; doxorubicin resistance; ecotropic viral integration site 32; MDR1A; multi-drug resistance 3; multidrug resistance p-glycoprotein; Multidrug resistance protein 1; Multidrug resistance protein 1A; Multidrug resistance protein 1B; Multidrug resistance protein 3; multiple drug resistant 1a; P glycoprotein 1; P glycoprotein 3; P-glycoprotein 1; P-glycoprotein 3; P-glycoprotein/multidrug resistance 1; Phospholipid transporter ABCB1
Gene Aliases: ABC20; ABCB1; Abcb1a; Abcb1b; Abcb4; CD243; CLCS; Evi32; GP170; mdr; mdr-3; MDR1; Mdr1a; Mdr1b; Mdr3; P-GP; Pgp; Pgy-1; Pgy-3; PGY1; Pgy1-1; Pgy3
UniProt ID: (Human) P08183, (Mouse) P21447, (Mouse) P06795
Entrez Gene ID: (Dog) 403879, (Human) 5243, (Mouse) 18671, (Rat) 170913, (Mouse) 18669, (Rat) 24646
transporter activity
protein binding
ATP binding
xenobiotic-transporting ATPase activity
ATPase activity, coupled to transmembrane movement of substances
nucleotide binding
hydrolase activity
ATPase activity
ATPase activity, coupled
drug transmembrane transporter activity
ATP-binding cassette (ABC) transporter
G2/M transition of mitotic cell cycle
antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent
positive regulation of antigen processing and presentation of peptide antigen via MHC class I
transport
drug transmembrane transport
response to drug
xenobiotic transport
transmembrane transport
stem cell proliferation
hormone transport
drug transport
daunorubicin transport
drug export
negative regulation of cell death
establishment of blood-brain barrier
positive regulation of response to drug
response to hypoxia
placenta development
lactation
circadian rhythm
metabolic process
response to organic cyclic compound
response to nutrient levels
response to vitamin D
cellular response to mycotoxin
response to cadmium ion
cellular response to antibiotic
cellular response to alkaloid
cellular response to estradiol stimulus
cellular response to organic cyclic compound
cellular hyperosmotic salinity response
response to dexamethasone
cellular response to dexamethasone stimulus
response to thyroxine
response to L-glutamate
establishment of endothelial blood-brain barrier
response to ionizing radiation
response to arsenic-containing substance
response to steroid hormone
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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