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Invitrogen
P-Selectin (CD62P) Polyclonal Antibody
FIGURE: 1 / 3
P-Selectin (CD62P) Antibody (PA5-79973) in WB
Western blot analysis of P-Selectin (CD62P) in Lane 1: mouse heart tissue lysate, Lane 2: rat heart tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with P-Selectin (CD62P) polyclonal antibody (Product # PA5-79973) at a dilution of 0.5 µg/mL (overnight... View More
Product Details
PA5-79973
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse CD62P recombinant protein (Position: W42-A267).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2747088
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; Granule membrane protein 140; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; RP1-86F14.2; selectin, platelet
Gene Aliases: CD62P; GMP-140; Grmp; LECAM3; PADGEM; PSELECT; Selp
UniProt ID: (Rat) P98106, (Mouse) Q01102
Entrez Gene ID: (Rat) 25651, (Mouse) 20344
positive regulation of leukocyte migration
regulation of cellular extravasation
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
positive regulation of platelet activation
positive regulation of phosphatidylinositol 3-kinase signaling
response to organic cyclic compound
single organismal cell-cell adhesion
response to lipopolysaccharide
regulation of integrin activation
positive regulation of cell adhesion
blood vessel morphogenesis
leukocyte migration
leukocyte tethering or rolling
cellular response to interleukin-6
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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