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P2X3 Antibody (PA5-115707) in ICC/IF
Immunocytochemistry analysis of P2X3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115707) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115707
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human P2RX3(Accession P56373), corresponding to amino acid residues D379-H397.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900342
Product Specific Information
Antibody detects endogenous levels of total P2X3.
Target Information
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and may transduce ATP-evoked nociceptor activation. Mouse studies suggest that this receptor is important for peripheral pain responses, and also participates in pathways controlling urinary bladder volume reflexes. It is possible that the development of selective antagonists for this receptor may have a therapeutic potential in pain relief and in the treatment of disorders of urine storage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP receptor; MGC129956; P2X purinoceptor 3; p2X receptor 3; P2X receptor, subunit 3; P2X3; p2X3 purinoceptor; p2xr3; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 3; purinergic receptor P2X, ligand-gated ion channel, 3; purinergic receptor P2X3; purinoceptor P2X3
Gene Aliases: 4930513E20Rik; P2RX3; P2X3
UniProt ID: (Human) P56373, (Rat) P49654, (Mouse) Q3UR32
Entrez Gene ID: (Human) 5024, (Rat) 81739, (Mouse) 228139
response to hypoxia
transport
signal transduction
neuromuscular synaptic transmission
blood coagulation
response to heat
response to cold
response to mechanical stimulus
response to carbohydrate
positive regulation of calcium ion transport into cytosol
urinary bladder smooth muscle contraction
neuronal action potential
peristalsis
response to ATP
purinergic nucleotide receptor signaling pathway
regulation of synaptic plasticity
positive regulation of calcium-mediated signaling
sensory perception of taste
protein homooligomerization
behavioral response to formalin induced pain
cation transmembrane transport
positive regulation of sensory perception of pain
ion transport
cation transport
chemical synaptic transmission
response to temperature stimulus
response to organic substance
behavioral response to pain
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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