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P2X4 Antibody (PA5-37880) in ICC/IF
Immunocytochemistry analysis of P2X4 using P2X4 Polyclonal Antibody (Product # PA5-37880) in paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL).
Product Details
PA5-37880
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-ETDSVVSSVTTKAK, from the internal region of mouse P2rx4
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2554488
Product Specific Information
This antibody is predicted to react with mouse, rat, dog, pig and cow based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP receptor; ATP-gated cation channel protein; P2X purinoceptor 4; P2X receptor, subunit 4; P2X4; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 4; purinergic receptor P2X, ligand-gated ion channel, 4; purinergic receptor P2X4; purinoceptor P2X4
Gene Aliases: P2RX4; P2X4; P2X4R
UniProt ID: (Human) Q99571
Entrez Gene ID: (Human) 5025
tissue homeostasis
regulation of sodium ion transport
transport
signal transduction
blood coagulation
regulation of blood pressure
positive regulation of calcium ion transport into cytosol
negative regulation of cardiac muscle hypertrophy
neuronal action potential
sensory perception of pain
positive regulation of prostaglandin secretion
response to ATP
ion transmembrane transport
response to fluid shear stress
purinergic nucleotide receptor signaling pathway
endothelial cell activation
positive regulation of nitric oxide biosynthetic process
positive regulation of calcium-mediated signaling
protein homooligomerization
membrane depolarization
positive regulation of calcium ion transport
regulation of cardiac muscle contraction
relaxation of cardiac muscle
cellular response to ATP
apoptotic signaling pathway
cation transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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