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P2X4 Receptor (extracellular) Antibody (APR-024-200UL) in ICC/IF
Expression of P2RX4 in rat pheochromocytoma cell line (PC12) - Cell surface detection of P2RX4 in intact living PC12 cells. A. Extracellular staining of cells with Anti-P2X4 (extracellular) Antibody (#APR-024), (red, 1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. Nuclear staining of cells using DAPI (blue). C. Merged images of A and B.
Product Details
APR-024-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RDLAGKEQRTLTK, corresponding to amino acid residues 301-313 of rat P2X4 receptor, Extracellular
Conjugate
Form
Lyophilized
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
Target Information
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP receptor; ATP-gated cation channel protein; ionotropic purinergic receptor; P2X purinoceptor 4; P2X receptor, subunit 4; P2X4; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 4; purinergic receptor P2X, ligand-gated ion channel, 4; purinergic receptor P2X4; purinergic receptor P2x4 subunit variant e; purinoceptor P2X4
Gene Aliases: AI504491; AW555605; D5Ertd444e; P2RX4; P2X4; P2X4R
UniProt ID: (Human) Q99571, (Rat) P51577
Entrez Gene ID: (Human) 5025, (Mouse) 18438, (Rat) 29659
tissue homeostasis
regulation of sodium ion transport
transport
signal transduction
blood coagulation
regulation of blood pressure
positive regulation of calcium ion transport into cytosol
negative regulation of cardiac muscle hypertrophy
neuronal action potential
sensory perception of pain
positive regulation of prostaglandin secretion
response to ATP
ion transmembrane transport
response to fluid shear stress
purinergic nucleotide receptor signaling pathway
endothelial cell activation
positive regulation of nitric oxide biosynthetic process
positive regulation of calcium-mediated signaling
protein homooligomerization
membrane depolarization
positive regulation of calcium ion transport
regulation of cardiac muscle contraction
relaxation of cardiac muscle
cellular response to ATP
apoptotic signaling pathway
cation transmembrane transport
nitric oxide biosynthetic process
ion transport
calcium ion transport
vasodilation
excitatory postsynaptic potential
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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