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p38 MAPK gamma Antibody (MA1-100) in IHC (P)
Immunohistochemistry analysis of MAPK12 showing positive staining in the nucleus and cytoplasm of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-... View More
Product Details
MA1-100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
10E1
Immunogen
Protein expressed in 293T cell transfected with human MAPK12 expression vector
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536744
Product Specific Information
MA1-100 has been successfully used in Western blot and immunohistochemistry applications and reacts with human and mouse samples.
MA1-100 specifically recognizes p38-gamma, and does not react with alpha, beta, or delta isoforms.
Target Information
MAPK12 (p38 gamma) is a serine/threonine kinase involved in many different cellular processes. It is activated during hypoxia and gamma-irradiation-induced G(2) arrest. Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. MAPK12 functions as a signal transducer during differentiation of myoblasts to myotubes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ERK-6; ERK5; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK 12; mitogen activated protein kinase 12; Mitogen-activated protein kinase 12; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; OTTHUMP00000196671; stress activated protein kinase 3; Stress-activated protein kinase 3
Gene Aliases: AW123708; ERK-6; ERK3; ERK6; MAPK 12; MAPK12; P38GAMMA; PRKM12; SAPK-3; SAPK3
UniProt ID: (Human) P53778, (Mouse) O08911
Entrez Gene ID: (Human) 6300, (Dog) 607023, (Mouse) 29857
magnesium ion binding
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
MAPK cascade
transcription, DNA-templated
regulation of transcription, DNA-templated
DNA damage induced protein phosphorylation
cell cycle arrest
signal transduction
muscle organ development
positive regulation of peptidase activity
peptidyl-serine phosphorylation
myoblast differentiation
vascular endothelial growth factor receptor signaling pathway
positive regulation of muscle cell differentiation
protein phosphorylation
cell cycle
phosphorylation
negative regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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