Invitrogen

PDHA1 Monoclonal Antibody (9H9AF5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

19 References

View all (32) PDHA1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PDHA1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

PDHA1 Antibody (459400) in ICC/IF

Immunofluorescence analysis of PDH was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PDHA1 Monoclonal Antibody (9H9AF5) (Product # 459400) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32733, ... View More

PDHA1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

PDHA1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK 293 cells were transfected with PDH siRNA and decrease in signal intensity was observed in Western blot application using Anti-PDHA1 Monoclonal Antibody (9H9AF5) (Product # 459400). {KD}

Product Details

459400

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

1 µg/mL

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

9H9AF5

Immunogen

Human recombinant full-length PDH E1-alpha

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_10375326

Product Specific Information

For positive control, use isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; cultured, normal Human embryonic lung fibroblasts (strain MRC5); HL60 cells.

Target Information

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chaperone-mediated autophagy prevents collapse of the neuronal metastable proteome.

Cell

Bourdenx M,Martín-Segura A,Scrivo A,Rodriguez-Navarro JA,Kaushik S,Tasset I,Diaz A,Storm NJ,Xin Q,Juste YR,Stevenson E,Luengo E,Clement CC,Choi SJ,Krogan NJ,Mosharov EV,Santambrogio L,Grueninger F,Collin L,Swaney DL,Sulzer D,Gavathiotis E,Cuervo AM

459400 was used in Flow cytometry/Cell sorting to conclude that functional CMA is essential for neuronal proteostasis through the maintenance of a subset of the proteome with a higher risk of misfolding than the general proteome.

Thu May 13 00:00:00 EDT 2021

Low-load resistance training to task failure with and without blood flow restriction: muscular functional and structural adaptations.

American journal of physiology. Regulatory, integrative and comparative physiology

Pignanelli C,Petrick HL,Keyvani F,Heigenhauser GJF,Quadrilatero J,Holloway GP,Burr JF

Published figure using PDHA1 monoclonal antibody (Product # 459400) in Western Blot

Sat Feb 01 00:00:00 EST 2020

Low-load resistance training to task failure with and without blood flow restriction: muscular functional and structural adaptations.

American journal of physiology. Regulatory, integrative and comparative physiology

Pignanelli C,Petrick HL,Keyvani F,Heigenhauser GJF,Quadrilatero J,Holloway GP,Burr JF

Published figure using PDHA1 monoclonal antibody (Product # 459400) in Western Blot

Sat Feb 01 00:00:00 EST 2020

Mitochondrial fusion supports increased oxidative phosphorylation during cell proliferation.

eLife

Yao CH,Wang R,Wang Y,Kung CP,Weber JD,Patti GJ

459400 was used in Western Blotting to reveal that cell proliferation requires increased OXPHOS as supported by mitochondrial fusion.

Tue Jan 29 00:00:00 EST 2019

Inhibition of ERRα Prevents Mitochondrial Pyruvate Uptake Exposing NADPH-Generating Pathways as Targetable Vulnerabilities in Breast Cancer.

Cell reports

Park S,Safi R,Liu X,Baldi R,Liu W,Liu J,Locasale JW,Chang CY,McDonnell DP

459400 was used in Western Blotting to show that ERRα inhibition interferes with pyruvate entry into mitochondria by inhibiting the expression of mitochondrial pyruvate carrier 1.

Tue Jun 18 00:00:00 EDT 2019

Exploring the In Vivo Role of the Mitochondrial Calcium Uniporter in Brown Fat Bioenergetics.

Cell reports

Flicker D,Sancak Y,Mick E,Goldberger O,Mootha VK

459400 was used in Western Blotting to study the effect of uniporter current on brown adipose tissue physiology.

Tue Apr 30 00:00:00 EDT 2019

Compartmentalized activities of the pyruvate dehydrogenase complex sustain lipogenesis in prostate cancer.

Nature genetics

Chen J,Guccini I,Di Mitri D,Brina D,Revandkar A,Sarti M,Pasquini E,Alajati A,Pinton S,Losa M,Civenni G,Catapano CV,Sgrignani J,Cavalli A,D'Antuono R,Asara JM,Morandi A,Chiarugi P,Crotti S,Agostini M,Montopoli M,Masgras I,Rasola A,Garcia-Escudero R,Delaleu N,Rinaldi A,Bertoni F,Bono J,Carracedo A,Alimonti A

Published figure using PDH monoclonal antibody (Product # 459400) in Immunofluorescence

Thu Feb 01 00:00:00 EST 2018

Compartmentalized activities of the pyruvate dehydrogenase complex sustain lipogenesis in prostate cancer.

Nature genetics

Published figure using PDH monoclonal antibody (Product # 459400) in Immunofluorescence

Thu Feb 01 00:00:00 EST 2018

Post-translational regulation of metabolism in fumarate hydratase deficient cancer cells.

Metabolic engineering

Gonçalves E,Sciacovelli M,Costa ASH,Tran MGB,Johnson TI,Machado D,Frezza C,Saez-Rodriguez J

459400 was used in Immunohistochemistry to design and acquire proteomics, phosphoproteomics, and metabolomics experiments in fumarate hydratase (FH) deficient cells and develop a computational modelling approach to identify putative regulatory phosphorylation-sites of metabolic enzymes.

Mon Jan 01 00:00:00 EST 2018

Sex differences in mitochondrial respiratory function in human skeletal muscle.

American journal of physiology. Regulatory, integrative and comparative physiology

Miotto PM,McGlory C,Holloway TM,Phillips SM,Holloway GP

459400 was used in Western Blotting to determine whether mitochondrial respiratory kinetics differed between healthy young men and women in permeabilized skeletal muscle fibers.

Fri Jun 01 00:00:00 EDT 2018

Quantitative metabolic flux analysis reveals an unconventional pathway of fatty acid synthesis in cancer cells deficient for the mitochondrial citrate transport protein.

Metabolic engineering

Jiang L,Boufersaoui A,Yang C,Ko B,Rakheja D,Guevara G,Hu Z,DeBerardinis RJ

459400 was used in Western Blot to describe the metabolic consequences of CTP deficiency in cancer cells.

Fri Sep 01 00:00:00 EDT 2017

Pyruvate kinase M2 and the mitochondrial ATPase Inhibitory Factor 1 provide novel biomarkers of dermatomyositis: a metabolic link to oncogenesis.

Journal of translational medicine

Santacatterina F,Sánchez-Aragó M,Catalán-García M,Garrabou G,de Arenas CN,Grau JM,Cardellach F,Cuezva JM

459400 was used in western blot to examine whether proteins of energy metabolism can be used as biomarkers of inflammatory myopathies

Fri Feb 10 00:00:00 EST 2017

Reductive carboxylation supports redox homeostasis during anchorage-independent growth.

Nature

Jiang L,Shestov AA,Swain P,Yang C,Parker SJ,Wang QA,Terada LS,Adams ND,McCabe MT,Pietrak B,Schmidt S,Metallo CM,Dranka BP,Schwartz B,DeBerardinis RJ

459400 was used in western blot to study the support of redox homeostasis during anchorage-independent growth by reductive carboxylation

Thu Apr 14 00:00:00 EDT 2016

Mutant Kras copy number defines metabolic reprogramming and therapeutic susceptibilities.

Nature

Kerr EM,Gaude E,Turrell FK,Frezza C,Martins CP

459400 was used in western blot to elucidate the mechanisms by which KRAS mutations contribute to lung cancer.

Thu Mar 03 00:00:00 EST 2016

E4F1-mediated control of pyruvate dehydrogenase activity is essential for skin homeostasis.

Proceedings of the National Academy of Sciences of the United States of America

Goguet-Rubio P,Seyran B,Gayte L,Bernex F,Sutter A,Delpech H,Linares LK,Riscal R,Repond C,Rodier G,Kirsh O,Touhami J,Noel J,Vincent C,Pirot N,Pavlovic G,Herault Y,Sitbon M,Pellerin L,Sardet C,Lacroix M,Le Cam L

459400 was used in western blot to show that E4F1 regulates pyruvate metabolism to maintain skin homeostasis

Tue Sep 27 00:00:00 EDT 2016

Effects of Taurine Administration on Carbohydrate Metabolism in Skeletal Muscle during the Post-Exercise Phase.

Journal of nutritional science and vitaminology

Takahashi Y,Tamura Y,Matsunaga Y,Kitaoka Y,Terada S,Hatta H

459400 was used in western blot to report the effects of taurine administration on glycogen repletion and carbohydrate metabolism in the tibialis anterior muscle after endurance exercise

Fri May 12 00:00:00 EDT 2017

ERRα-Regulated Lactate Metabolism Contributes to Resistance to Targeted Therapies in Breast Cancer.

Cell reports

Park S,Chang CY,Safi R,Liu X,Baldi R,Jasper JS,Anderson GR,Liu T,Rathmell JC,Dewhirst MW,Wood KC,Locasale JW,McDonnell DP

Published figure using PDHA1 monoclonal antibody (Product # 459400) in Western Blot

Tue Apr 12 00:00:00 EDT 2016

Ras-mediated modulation of pyruvate dehydrogenase activity regulates mitochondrial reserve capacity and contributes to glioblastoma tumorigenesis.

Neuro-oncology

Prabhu A,Sarcar B,Miller CR,Kim SH,Nakano I,Forsyth P,Chinnaiyan P

459400 was used in western blot to discuss the metabolic programs that drive tumorigenesis.

Tue Sep 01 00:00:00 EDT 2015

Dichloroacetate induces apoptosis and cell-cycle arrest in colorectal cancer cells.

British journal of cancer

Madhok BM,Yeluri S,Perry SL,Hughes TA,Jayne DG

459400 was used in western blot to test if switching the metabolism from glycolysis towards mitochondrial respiration reduces the growth of colorectal cancer cells and explore the mechanism

Tue Jun 08 00:00:00 EDT 2010

Dichloroacetate induces apoptosis and cell-cycle arrest in colorectal cancer cells.

British journal of cancer

Madhok BM,Yeluri S,Perry SL,Hughes TA,Jayne DG

459400 was used in western blot to test if switching the metabolism from glycolysis towards mitochondrial respiration reduces the growth of colorectal cancer cells and explore the mechanism

Tue Jun 08 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: PDHE1-A type I; pyruvate dehydrogenase; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase alpha 1; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 alpha 1 subunit; pyruvate dehydrogenase E1 component alpha subunit; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; pyruvate dehydrogenase E1 subunit; pyruvate dehydrogenase E1alpha subunit

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Gene Aliases: PDHA; Pdha-1; PDHA1; PDHAD; PDHCE1A; PHE1A

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UniProt ID: (Human) P08559, (Bovine) A7MB35, (Mouse) P35486

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Entrez Gene ID: (Human) 5160, (Bovine) 407109, (Rat) 29554, (Mouse) 18597

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Function(s)

pyruvate dehydrogenase activity pyruvate dehydrogenase (acetyl-transferring) activity pyruvate dehydrogenase (NAD+) activity oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor dehydrogenase

Process(es)

glucose metabolic process acetyl-CoA biosynthetic process from pyruvate pyruvate metabolic process tricarboxylic acid cycle regulation of acetyl-CoA biosynthetic process from pyruvate glyoxylate metabolic process mitochondrial acetyl-CoA biosynthetic process from pyruvate glycolytic process oxidation-reduction process carbohydrate metabolic process metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PDHA1 Monoclonal Antibody (9H9AF5)

PDHA1 Antibody (459400) in ICC/IF

Product Details

459400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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