Proteintech

PFKP Polyclonal Antibody

PFKP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

PFKP Antibody (13389-1-AP) in ICC/IF

Immunofluorescent analysis of BxPC-3 cells using 13389-1-AP (PFKP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PFKP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PFKP Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13389-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PFKP Fusion Protein Ag4047 (436-784 aa encoded by BC029138)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GIADGHRML AIYDGFDGFA KGQIKEIGWT DVGGWTGQGG SILGTKRVLP GKYLEEIATQ MRTHSINALL IIGGFEAYLG LLELSAAREK HEEFCVPMVM VPATVSNNVP GSDFSIGADT ALNTITDTCD RIKQSASGTK RRVFIIETMG GYCGYLANMG GLAAGADAAY IFEEPFDIRD LQSNVEHLTE KMKTTIQRGL VLRNESCSEN YTTDFIYQLY SEEGKGVFDC RKNVLGHMQQ GGAPSPFDRN FGTKISARAM EWITAKLKEA RGRGKKFTTD DSICVLGISK RNVIFQPVAE LKKQTDFEHR IPKEQWWLKL RPLMKILAKY KASYDVSDSG QLEHVQPWSV (436-784 aa encoded by BC029138)

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

C11orf54 promotes DNA repair via blocking CMA-mediated degradation of HIF1A.

Communications biology

Tan J,Wang W,Liu X,Xu J,Che Y,Liu Y,Hu J,Hu L,Li J,Zhou Q

Mon Jun 05 00:00:00 EDT 2023

Glucose-6-phosphate dehydrogenase exerts antistress effects independently of its enzymatic activity.

The Journal of biological chemistry

Jin X,Li X,Li L,Zhong B,Hong Y,Niu J,Li B

Thu Dec 01 00:00:00 EST 2022

Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.

Acta pharmacologica Sinica

Xu L,Yang TY,Zhou YW,Wu MF,Shen J,Cheng JL,Liu QX,Cao SY,Wang JQ,Zhang L

Tue Feb 01 00:00:00 EST 2022

R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m⁶A/PFKP/LDHB axis.

Molecular cell

Qing Y,Dong L,Gao L,Li C,Li Y,Han L,Prince E,Tan B,Deng X,Wetzel C,Shen C,Gao M,Chen Z,Li W,Zhang B,Braas D,Ten Hoeve J,Sanchez GJ,Chen H,Chan LN,Chen CW,Ann D,Jiang L,Müschen M,Marcucci G,Plas DR,Li Z,Su R,Chen J

Thu Mar 04 00:00:00 EST 2021

RSL3 enhances the antitumor effect of cisplatin on prostate cancer cells via causing glycolysis dysfunction.

Biochemical pharmacology

Li M,Chen X,Wang X,Wei X,Wang D,Liu X,Xu L,Batu W,Li Y,Guo B,Zhang L

Fri Oct 01 00:00:00 EDT 2021

Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease.

Frontiers in pharmacology

Zhou Y,Wu M,Xu L,Cheng J,Shen J,Yang T,Zhang L

Fri Apr 02 00:00:00 EDT 2021

The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.

Cell death & disease

Gao W,Huang M,Chen X,Chen J,Zou Z,Li L,Ji K,Nie Z,Yang B,Wei Z,Xu P,Jia J,Zhang Q,Shen H,Wang Q,Li K,Zhu L,Wang M,Ye S,Zeng S,Lin Y,Rong Z,Xu Y,Zhu P,Zhang H,Hao B,Liu Q

Thu Apr 15 00:00:00 EDT 2021

Triptolide impairs glycolysis by suppressing GATA4/Sp1/PFKP signaling axis in mouse Sertoli cells.

Toxicology and applied pharmacology

Zhang Y,Tang Y,Luo Y,Luo L,Shen F,Huang Z

Sun Aug 15 00:00:00 EDT 2021

Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.

Oncology letters

Shi W,Fu Y,Wang Y

Thu Jul 01 00:00:00 EDT 2021

A long non-coding RNA specifically expressed in early embryos programs the metabolic balance in adult mice.

Biochimica et biophysica acta. Molecular basis of disease

Zhu M,Wang Q,Tian P,Cheng L,Sun Z,Hong Q,Lv P,Ji L,Liu Y,Tang QQ,Wen B

Fri Jan 01 00:00:00 EST 2021

Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis.

Frontiers in pharmacology

Chen Y,Chen J,Shu A,Liu L,Wu Q,Wu J,Song S,Fan W,Zhu Y,Xu H,Sun J,Yang L

Fri Jul 16 00:00:00 EDT 2021

Novel mitochondrion-targeting copper(II) complex induces HK2 malfunction and inhibits glycolysis via Drp1-mediating mitophagy in HCC.

Journal of cellular and molecular medicine

Li M,Shao J,Guo Z,Jin C,Wang L,Wang F,Jia Y,Zhu Z,Zhang Z,Zhang F,Zheng S,Wang X

Sun Mar 01 00:00:00 EST 2020

Novel mitochondrion-targeting copper(II) complex induces HK2 malfunction and inhibits glycolysis via Drp1-mediating mitophagy in HCC.

Journal of cellular and molecular medicine

Li M,Shao J,Guo Z,Jin C,Wang L,Wang F,Jia Y,Zhu Z,Zhang Z,Zhang F,Zheng S,Wang X

Sun Mar 01 00:00:00 EST 2020

Ginsenoside Rh4 suppresses aerobic glycolysis and the expression of PD-L1 via targeting AKT in esophageal cancer.

Biochemical pharmacology

Deng X,Zhao J,Qu L,Duan Z,Fu R,Zhu C,Fan D

Sat Aug 01 00:00:00 EDT 2020

Ginsenoside Rh4 suppresses aerobic glycolysis and the expression of PD-L1 via targeting AKT in esophageal cancer.

Biochemical pharmacology

Deng X,Zhao J,Qu L,Duan Z,Fu R,Zhu C,Fan D

Sat Aug 01 00:00:00 EDT 2020

Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.

The Journal of investigative dermatology

Gu Z,Zhang H,Guo X,Cao Y

Sat Aug 01 00:00:00 EDT 2020

The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.

Stem cell reports

Sun Z,Zhu M,Lv P,Cheng L,Wang Q,Tian P,Yan Z,Wen B

Tue Sep 11 00:00:00 EDT 2018

The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.

Stem cell reports

Sun Z,Zhu M,Lv P,Cheng L,Wang Q,Tian P,Yan Z,Wen B

Tue Sep 11 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: 6-phosphofructo-1-kinase, C-type; 6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; ATP-dependent 6-phosphofructokinase, platelet type; ATP-dependent 6-phosphofructokinase, platelet type {ECO:0000255|HAMAP-Rule:MF_03184}; ATP-PFK; ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}; ATP:D-fructose 6-phosphate 1-phosphotransferase; EC 2.7.1.11; K6PP; PFK 1; PFK-C; PFK1; Phosphofructo-1-kinase isozyme C; Phosphofructokinase; phosphofructokinase 1; Phosphofructokinase, platelet type; Phosphohexokinase; phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}

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Gene Aliases: 1200015H23Rik; 9330125N24Rik; ATP-PFK; PFK-C; PFK-P; Pfkc; PFKF; PFKP

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UniProt ID: (Human) Q01813, (Rat) P47860, (Mouse) Q9WUA3

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Entrez Gene ID: (Human) 5214, (Rat) 60416, (Mouse) 56421

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Function(s)

6-phosphofructokinase activity ATP binding protein complex binding metal ion binding cadherin binding involved in cell-cell adhesion fructose-6-phosphate binding nucleotide binding catalytic activity kinase activity transferase activity carbohydrate kinase kinase transferase metabolite interconversion enzyme

Process(es)

fructose 6-phosphate metabolic process canonical glycolysis cell-cell adhesion mitophagy in response to mitochondrial depolarization glycolytic process fructose 1,6-bisphosphate metabolic process carbohydrate phosphorylation protein homotetramerization metabolic process phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PFKP Polyclonal Antibody

PFKP Antibody (13389-1-AP) in ICC/IF

Product Details

13389-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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