Invitrogen

PGK1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

20 Published Figures

18 References

View all (19) PGK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PGK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 31

PGK1 Antibody (PA5-28612) in ICC/IF

Immunofluorescence analysis of PGK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PGK1 Polyclonal Antibody (Product # PA5-28612) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a d... View More

PGK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PGK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

PGK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PGK1 siRNA and loss of signal was observed in Western Blot using Anti-PGK 1 Polyclonal Antibody (Product # PA5-28612). {KD}

Product Details

PA5-28612

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 17 publications 17 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:20-1:1,000

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

in situ PLA (PLA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat, Yeast

Published species

Human, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 217 of Human PGK1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546088

Product Specific Information

Recommended positive controls: 293T, PGK1 shRNA-transfected 293T, A431, HeLa, HepG2, mouse brain, Rat liver, Yeast wild type 303.

Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (87%), Xenopus laevis (84%), Pig (96%), Chicken (88%), Sheep (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Also known as ATP:3-phosphoglycerate 1-phosphotransferase showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Xrn1 is a deNADding enzyme modulating mitochondrial NAD-capped RNA.

Nature communications

Sharma S,Yang J,Grudzien-Nogalska E,Shivas J,Kwan KY,Kiledjian M

PA5-28612 was used in Western Blot to uncover a contribution of mitochondrial NAD-capped RNAs in overall NAD regulation with the deNADding activity of Xrn1 fulfilling a central role.

Wed Feb 16 00:00:00 EST 2022

Xrn1 is a deNADding enzyme modulating mitochondrial NAD-capped RNA.

Nature communications

Sharma S,Yang J,Grudzien-Nogalska E,Shivas J,Kwan KY,Kiledjian M

PA5-28612 was used in Western Blot to uncover a contribution of mitochondrial NAD-capped RNAs in overall NAD regulation with the deNADding activity of Xrn1 fulfilling a central role.

Wed Feb 16 00:00:00 EST 2022

The C-terminal domain of Hsp70 is responsible for paralog-specific regulation of ribonucleotide reductase.

PLoS genetics

Knighton LE,Omkar S,Truman AW

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Western Blot

Fri Apr 01 00:00:00 EDT 2022

The Smk1 MAPK and Its Activator, Ssp2, Are Required for Late Prospore Membrane Development in Sporulating Saccharomyces cerevisiae.

Journal of fungi (Basel, Switzerland)

Durant M,Roesner JM,Mucelli X,Slubowski CJ,Klee E,Seitz BC,Wallis Z,Huang LS

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Western Blot

Thu Jan 14 00:00:00 EST 2021

The Smk1 MAPK and Its Activator, Ssp2, Are Required for Late Prospore Membrane Development in Sporulating Saccharomyces cerevisiae.

Journal of fungi (Basel, Switzerland)

Durant M,Roesner JM,Mucelli X,Slubowski CJ,Klee E,Seitz BC,Wallis Z,Huang LS

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Western Blot

Thu Jan 14 00:00:00 EST 2021

Cex1 is a component of the COPI intracellular trafficking machinery.

Biology open

Enkler L,Rinaldi B,de Craene JO,Hammann P,Nureki O,Senger B,Friant S,Becker HD

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Western Blot

Mon Mar 22 00:00:00 EDT 2021

Epigenetic CRISPR Screens Identify Npm1 as a Therapeutic Vulnerability in Non-Small Cell Lung Cancer.

Cancer research

Li F,Ng WL,Luster TA,Hu H,Sviderskiy VO,Dowling CM,Hollinshead KER,Zouitine P,Zhang H,Huang Q,Ranieri M,Wang W,Fang Z,Chen T,Deng J,Zhao K,So HC,Khodadadi-Jamayran A,Xu M,Karatza A,Pyon V,Li S,Pan Y,Labbe K,Almonte C,Poirier JT,Miller G,Possemato R,Qi J,Wong KK

PA5-28612 was used in Western Blotting to support NPM1 as a therapeutic vulnerability in NSCLC. SIGNIFICANCE: Epigenome-wide CRISPR knockout screens identify NPM1 as a novel metabolic vulnerability and demonstrate that targeting NPM1 is a new therapeutic opportunity for patients with NSCLC.

Tue Sep 01 00:00:00 EDT 2020

Mitochondrial-nuclear heme trafficking in budding yeast is regulated by GTPases that control mitochondrial dynamics and ER contact sites.

Journal of cell science

Martinez-Guzman O,Willoughby MM,Saini A,Dietz JV,Bohovych I,Medlock AE,Khalimonchuk O,Reddi AR

PA5-28612 was used in Western Blotting to indicate that there are parallel pathways for the distribution of bioavailable heme.

Wed May 20 00:00:00 EDT 2020

Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast.

Life science alliance

Fan J,Jin H,Koch BA,Yu HG

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Immunoprecipitation

Tue Dec 01 00:00:00 EST 2020

Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast.

Life science alliance

Fan J,Jin H,Koch BA,Yu HG

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Immunoprecipitation

Tue Dec 01 00:00:00 EST 2020

Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast.

Life science alliance

Fan J,Jin H,Koch BA,Yu HG

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Immunoprecipitation

Tue Dec 01 00:00:00 EST 2020

Andrographolide and Its 14-Aryloxy Analogues Inhibit Zika and Dengue Virus Infection.

Molecules (Basel, Switzerland)

Li F,Khanom W,Sun X,Paemanee A,Roytrakul S,Wang D,Smith DR,Zhou GC

PA5-28612 was used in Western Blotting to suggest that 14-aryloxy analogues of andrographolide have the potential for further development as anti-DENV and anti-ZIKV agents.

Fri Oct 30 00:00:00 EDT 2020

Andrographolide and Its 14-Aryloxy Analogues Inhibit Zika and Dengue Virus Infection.

Molecules (Basel, Switzerland)

Li F,Khanom W,Sun X,Paemanee A,Roytrakul S,Wang D,Smith DR,Zhou GC

PA5-28612 was used in Western Blotting to suggest that 14-aryloxy analogues of andrographolide have the potential for further development as anti-DENV and anti-ZIKV agents.

Fri Oct 30 00:00:00 EDT 2020

The anaphase-promoting complex regulates the degradation of the inner nuclear membrane protein Mps3.

The Journal of cell biology

Koch BA,Jin H,Tomko RJ,Yu HG

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Western Blot

Mon Mar 04 00:00:00 EST 2019

Molecular interactions between Hel2 and RNA supporting ribosome-associated quality control.

Nature communications

Winz ML,Peil L,Turowski TW,Rappsilber J,Tollervey D

PA5-28612 was used in Western Blotting to demonstrate a ribosome-associated quality control molecular mechanism, in which Hel2 associates with ribosomal subunits and scans ribosome-mRNA complexes.

Mon Feb 04 00:00:00 EST 2019

Complex ATP7B mutation patterns in Wilson disease and evaluation of a yeast model for functional analysis of variants.

Human mutation

Li X,Zhang W,Zhou D,Lv T,Xu A,Wang H,Zhao X,Zhang B,Li Y,Jia S,Wang Y,Wang X,Wu Z,Duan W,Wang Q,Nan Y,Shang J,Jiang W,Chen Y,Zheng S,Liu M,Sun L,You H,Jia J,Ou X,Huang J

PA5-28612 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Proximity Ligation Assay, Western Blot to reveal the complex ATP7B mutation patterns in WD patients and the applicability of a yeast model system to the evaluation of the functional consequences of ATP7B variants, which is essential for WD cases that are difficult to interpret.

Wed May 01 00:00:00 EDT 2019

Extra-mitochondrial Cu/Zn superoxide dismutase (Sod1) is dispensable for protection against oxidative stress but mediates peroxide signaling in Saccharomyces cerevisiae.

Redox biology

Montllor-Albalate C,Colin AE,Chandrasekharan B,Bolaji N,Andersen JL,Wayne Outten F,Reddi AR

PA5-28612 was used in Western Blotting to show that the vast majority of Sod1 in yeast is utilised for peroxide-mediated signalling rather than superoxide scavenging.

Fri Feb 01 00:00:00 EST 2019

Nuclear RNA Decay Pathways Aid Rapid Remodeling of Gene Expression in Yeast.

Molecular cell

Bresson S,Tuck A,Staneva D,Tollervey D

PA528612 was used in western blot to examine the role of nuclear surveillance in reprogramming gene expression in yeast

Thu Mar 02 00:00:00 EST 2017

Cost-effective and rapid lysis of Saccharomyces cerevisiae cells for quantitative western blot analysis of proteins, including phosphorylated eIF2α.

Yeast (Chichester, England)

Lee SJ,Ramesh R,de Boor V,Gebler JM,Silva RC,Sattlegger E

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Western Blot

Fri Sep 01 00:00:00 EDT 2017

The Assembly Factor Pet117 Couples Heme a Synthase Activity to Cytochrome Oxidase Assembly.

The Journal of biological chemistry

Taylor NG,Swenson S,Harris NJ,Germany EM,Fox JL,Khalimonchuk O

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Western Blot

Fri Feb 03 00:00:00 EST 2017

The Assembly Factor Pet117 Couples Heme a Synthase Activity to Cytochrome Oxidase Assembly.

The Journal of biological chemistry

Taylor NG,Swenson S,Harris NJ,Germany EM,Fox JL,Khalimonchuk O

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Western Blot

Fri Feb 03 00:00:00 EST 2017

Cleavage of the SUN-domain protein Mps3 at its N-terminus regulates centrosome disjunction in budding yeast meiosis.

PLoS genetics

Li P,Jin H,Koch BA,Abblett RL,Han X,Yates JR,Yu HG

Published figure using PGK1 polyclonal antibody (Product # PA5-28612) in Western Blot

Thu Jun 01 00:00:00 EDT 2017

Unrestrained AMPylation targets cytosolic chaperones and activates the heat shock response.

Proceedings of the National Academy of Sciences of the United States of America

Truttmann MC,Zheng X,Hanke L,Damon JR,Grootveld M,Krakowiak J,Pincus D,Ploegh HL

PA5-28612 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Proximity Ligation Assay, Western Blot to suggest a mode of Hsp70 inactivation by AMPylation and point toward a role for protein AMPylation in the regulation of cellular protein homeostasis beyond the endoplasmic reticulum.

Tue Jan 10 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p; Phosphoglycerate kinase; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2

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Gene Aliases: HEL-S-68p; MIG10; OK/SW-cl.110; Pgk; Pgk-1; PGK1; PGKA; YCR012W; YCR12W

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UniProt ID: (Human) P00558, (Baker's yeast) P00560, (Mouse) P09411, (Rat) P16617

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Entrez Gene ID: (Human) 5230, (Baker's yeast) 850370, (Mouse) 18655, (Rat) 24644

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Function(s)

phosphoglycerate kinase activity protein binding ATP binding nucleotide binding kinase activity transferase activity ADP binding carbohydrate kinase

Process(es)

gluconeogenesis phosphorylation epithelial cell differentiation canonical glycolysis glycolytic process carbohydrate metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PGK1 Polyclonal Antibody

PGK1 Antibody (PA5-28612) in ICC/IF

Product Details

PA5-28612

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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