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Invitrogen
Paxillin Recombinant Rabbit Monoclonal Antibody (ARC0368)
FIGURE: 1 / 2
Paxillin Antibody (MA5-35261) in WB
Western blot analysis of Paxillin in extracts of various cell lines. Samples were incubated with Paxillin Monoclonal antibody (Product # MA5-35261) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-35261
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0368
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Paxillin (P49023)
Conjugate
Form
Liquid
Concentration
0.15 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849164
Product Specific Information
Immunogen sequence: MDDLDALLAD LESTTSHISK RPVFLSEETP YSYPTGNHTY QEIAVPPPVP PPPSSEALNG TILDPLDQWQ PSSSRFIHQQ PQSSSPVYGS SAKTSSVSNP
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ16691; hCG_1778014; myocardial ischemic preconditioning associated protein 7; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134
Gene Aliases: AW108311; AW123232; Pax; PXN
UniProt ID: (Human) P49023, (Mouse) Q8VI36
Entrez Gene ID: (Human) 5829, (Mouse) 19303, (Rat) 360820
muscle contraction
cell adhesion
cell-matrix adhesion
signal transduction
signal complex assembly
epidermal growth factor receptor signaling pathway
transforming growth factor beta receptor signaling pathway
cellular response to reactive oxygen species
vascular endothelial growth factor receptor signaling pathway
growth hormone receptor signaling pathway
activation of MAPK activity
movement of cell or subcellular component
cytoskeleton organization
integrin-mediated signaling pathway
regulation of cell shape
peptidyl-tyrosine phosphorylation
lamellipodium assembly
substrate adhesion-dependent cell spreading
positive regulation of protein kinase activity
focal adhesion assembly
branching morphogenesis of an epithelial tube
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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