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Invitrogen
Paxillin Chimeric Recombinant Mouse Monoclonal Antibody (LD2 sAB)
FIGURE: 1 / 1
Paxillin Chimeric Antibody (MA5-47943) in WB
Western Blot analysis of Paxillin was performed using Paxillin Recombinant Monoclonal Antibody (Product # MA5-47943). HepG2 (A), A431 (B), and HeLa (C) cell lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with Paxillin Recombinant Monoclonal Antibody at 2 µg/mL before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Paxillin is 64.5 kDa. Paxillin Recombinant Monoclonal Antibod... View More
Product Details
MA5-47943
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
LD2 sAB
Immunogen
Peptide corresponding to paxillin-LD2 motif (residues 141-156) fused to MBP; peptide sequence: NLSELDRLLLELNAVQHNP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074387
Product Specific Information
Specificity: The antibody binds specifically to the LD-2 motif of human paxillin with a Kd of 24 nM.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ16691; hCG_1778014; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134
Gene Aliases: PXN
UniProt ID: (Human) P49023
Entrez Gene ID: (Human) 5829
muscle contraction
cell adhesion
cell-matrix adhesion
signal transduction
signal complex assembly
epidermal growth factor receptor signaling pathway
transforming growth factor beta receptor signaling pathway
cellular response to reactive oxygen species
vascular endothelial growth factor receptor signaling pathway
growth hormone receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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