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Phospho-BACH1 (Ser445) Antibody (PA5-105364) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-BACH1 (Ser445) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-BACH1 (Ser445) polyclonal antibody (Product # PA5-105364) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105364
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BACH1(Accession O14867), corresponding to amino acid residues around phosphorylated Ser445.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816792
Product Specific Information
Antibody detects endogenous levels of BACH1 only when phosphorylated at Ser445.
Target Information
BACH1.3 is a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo and/or hetero oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. Some exons of this gene overlap with some exons from the C21orf41 gene, which is transcribed in an opposite orientation to this gene, but which does not seem to encode a protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homolog 1; BTB and CNC homology 1 transcription factor; BTB and CNC homology 1, basic leucine zipper transcription factor 1; HA2303; Transcription regulator protein BACH1
Gene Aliases: 6230421P05Rik; AI323795; BACH-1; BACH1; BTBD24
UniProt ID: (Human) O14867, (Mouse) P97302
Entrez Gene ID: (Human) 571, (Mouse) 12013, (Rat) 304127
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription factor activity, sequence-specific DNA binding
ubiquitin-protein transferase activity
protein binding
heme binding
DNA binding
sequence-specific DNA binding
regulation of transcription involved in G1/S transition of mitotic cell cycle
regulation of transcription involved in G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
DNA repair
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter in response to hypoxia
protein ubiquitination
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