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Phospho-BAG3 (Tyr457) Antibody (PA5-105968) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-BAG3 (Tyr457) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-BAG3 (Tyr457) polyclonal antibody (Product # PA5-105968) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105968
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BAG3(Accession O95817), corresponding to amino acid residues around phosphorylated Tyr457.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817367
Product Specific Information
Antibody detects endogenous levels of BAG3 only when phosphorylated at Tyr457.
Target Information
BAG3 are among BAG proteins that compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Bcl-2-interacting death suppressor; BCL2-associated athanogene 3; BCL2-binding athanogene 3; DKFZp434E0610; Docking protein CAIR-1; MGC104307
Gene Aliases: AA407278; BAG-3; BAG3; BIS; CAIR-1; MFM6; mg638; MNCb-2243
UniProt ID: (Human) O95817, (Mouse) Q9JLV1
Entrez Gene ID: (Human) 9531, (Mouse) 29810, (Rat) 293524
apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of striated muscle cell apoptotic process
protein stabilization
cellular response to mechanical stimulus
extrinsic apoptotic signaling pathway in absence of ligand
brain development
spinal cord development
protein folding
negative regulation of apoptotic process
regulation of catalytic activity
cell-cell adhesion
regulation of cellular response to heat
Performance Guarantee
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