Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
Phospho-DAPK1 (Ser308) Antibody (MA1-24738) in WB
Western blot analysis of Dapk1 Ser308ph was performed by loading whole cell lysate (in 1X SDS sample buffer with 2-ME) from 2 x 105 mouse embryonic fibroblasts. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # WG1402BOX). Proteins were transferred to nitrocellulose membrane by wet/tank transfer. Membrane was blocked in 5% milk/TBST and probed with a monoclonal anti-pDapk1 (S308) anti... View More
Product Details
MA1-24738
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
DKPS308
Immunogen
Synthetic phosphopeptide corresponding to residues 303-312 of human DAP Kinase 1.
Conjugate
Form
Liquid
Concentration
2.2 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2090373
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DAPK1 is a large, calmodulin-dependent kinase involved in gamma-interferon induced programmed cell death and is a potential tumor suppressor. DAPK1 is involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and authophagy. It regulates both type I apoptotic and type II autophagic cell death signal, depending on the cellular setting. Type I apoptotic cell death is caspase-dependent, type II authophagic cell death is caspase-independent. DAPK1 phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. It also phosphorylates TPM1, STX1A, PRKD1, BECN1, TSC2, RPS6, MYL9, and DAPK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DAP kinase 1; Death-associated protein kinase 1; DKFZp781I035; OTTHUMP00000216193
Gene Aliases: D13Ucla1; DAP-Kinase; DAPK; DAPK1
UniProt ID: (Human) P53355, (Mouse) Q80YE7
Entrez Gene ID: (Human) 1612, (Mouse) 69635
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
protein binding
calmodulin binding
ATP binding
GTP binding
syntaxin-1 binding
identical protein binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein phosphorylation
apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
regulation of autophagy
negative regulation of translation
intracellular signal transduction
regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein autophosphorylation
cellular response to interferon-gamma
apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of N-methyl-D-aspartate selective glutamate receptor activity
regulation of translation
signal transduction
phosphorylation
negative regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support