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Phospho-DOK1 (Ser450) Antibody (PA5-143682) in WB
Western blot of Phospho-DOK1 (Ser450) in Jurkat cells stimulated with calyculin A (100 nM, 30 min.) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), anti-Dok1 (Tyr362) (lanes 3 & 4), and Phospho-DOK1 (Ser450) polyclonal antibody (Product # PA5-143682) (lanes 5 & 6).
Product Details
PA5-143682
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-Dok1 (Ser-450) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 450 in human Dok1. This sequence is conserved in Dok1 from rat (Ser-449) and mouse (Ser-451). The site is not conserved in other Dok family members.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942910
Product Specific Information
This antibody was cross-adsorbed to an unphosphorylated Dok1 (Ser-450) peptide before affinity purification using phospho-Dok1 (Ser-450) peptide. The purified antibody detects a band at 62 kDa* corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after lambda phosphatase treatment. Similar to Dok1 (Tyr-362) antibody, this antibody also detects an 80 kDa band that has not been identified.
Target Information
DOK1 (P62dok, Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 is a major substrate for many tyrosine kinases including c-kit, v-abl, vFPS, EGF, and PDGF. Upon phosphorylation by kinases, DOK1 forms a complex with ras GTPase-activating protein. Phosphorylation on tyrosine residues by the insulin receptor kinase results in the negative regulation of the insulin signaling pathway. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Docking protein 1; docking protein 1, 62kDa (downstream of tyrosine kinase 1); DOK 1; Downstream of tyrosine kinase 1; downstream of tyrosine kinase-1; MGC117395; MGC138860; p62 dok; p62(dok); pp62; TLP1; TP1; TROVE domain family; TROVE1; VAULT2
Gene Aliases: AW557123; Dok; Dok-1; DOK1; P62DOK; pp62
UniProt ID: (Human) Q9UHG6, (Rat) Q4QQV2, (Mouse) P97465
Entrez Gene ID: (Human) 1796, (Rat) 312477, (Mouse) 13448
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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