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Invitrogen
Phospho-DYRK2/DYRK4 (Tyr382, Tyr264) Polyclonal Antibody
FIGURE: 1 / 4
Phospho-DYRK2/DYRK4 (Tyr382, Tyr264) Antibody (PA5-105875) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-DYRK2/DYRK4 (Tyr386, Tyr268) in rat intestinal tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-DYRK2/DYRK4 (Tyr386, Tyr268) polyclonal antibody (Product # PA5-105875) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-r... View More
Product Details
PA5-105875
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DYRK2(Accession Q92630), corresponding to amino acid residues around phosphorylated Tyr382.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817274
Product Specific Information
Antibody detects endogenous levels of DYRK2/4 only when phosphorylated at Y382/Y264.
Target Information
DYRK2 is a member of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family that is thought to be involved in cellular proliferation and apoptosis. Under normal conditions, nuclear but not cytoplasmic DYRK2 is ubiquitinated by MDM2, leading to its constitutive degradation. However, upon exposure to genotoxic stress, ATM phosphorylates DYRK2, leading to its dissociation from MDM2 and its phosphorylation of p53, thereby inducing apoptosis. Recent evidence also suggests that DYRK2 may serve as a scaffold that facilitates assembly of an E3 ubiquitin ligase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dual specificity tyrosine-(Y)-phosphorylation regulated kinase 2; dual specificity tyrosine-(Y)-phosphorylation regulated kinase 4; Dual specificity tyrosine-phosphorylation-regulated kinase 2; Dual specificity tyrosine-phosphorylation-regulated kinase 4; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4; dyrk2; dyrk4; RAD51 associated protein 1
Gene Aliases: 1810038L18Rik; AW049118; DYRK2; DYRK4; Dyrk4a; Dyrk4b; Rad51ap1
UniProt ID: (Human) Q92630, (Human) Q9NR20, (Mouse) Q5U4C9, (Mouse) Q8BI55
Entrez Gene ID: (Human) 8445, (Human) 8798, (Mouse) 69181, (Rat) 314862, (Mouse) 101320, (Rat) 312721
magnesium ion binding
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
manganese ion binding
ubiquitin binding
metal ion binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein phosphorylation
apoptotic process
smoothened signaling pathway
phosphorylation
peptidyl-tyrosine phosphorylation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of glycogen biosynthetic process
negative regulation of NFAT protein import into nucleus
cellular response to DNA damage stimulus
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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