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Phospho-EEF2 (Tyr443) Antibody (PA5-105490) in ICC/IF
Immunocytochemistry analysis of Phospho-EEF2 (Tyr443) in H2O2 treated HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-EEF2 (Tyr443) polyclonal antibody (Product # PA5-105490) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (... View More
Product Details
PA5-105490
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EEF2(Accession P13639), corresponding to amino acid residues around phosphorylated Tyr443.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816918
Product Specific Information
Antibody detects endogenous levels of EEF2 only when phosphorylated at Tyr443.
Target Information
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EF-2; Elongation factor 2; elongation factor-2; polypeptidyl-tRNA translocase
Gene Aliases: EEF-2; EEF2; EF-2; EF2; SCA26
UniProt ID: (Human) P13639, (Mouse) P58252, (Rat) P05197
Entrez Gene ID: (Human) 1938, (Mouse) 13629, (Rat) 29565
p53 binding
translation elongation factor activity
GTPase activity
protein binding
GTP binding
5S rRNA binding
protein kinase binding
ribosome binding
poly(A) RNA binding
actin filament binding
cadherin binding involved in cell-cell adhesion
nucleotide binding
translation activator activity
translation elongation factor
hematopoietic progenitor cell differentiation
response to ischemia
skeletal muscle contraction
translational elongation
aging
glial cell proliferation
response to fluoxetine
peptidyl-diphthamide biosynthetic process from peptidyl-histidine
response to estradiol
response to endoplasmic reticulum stress
skeletal muscle cell differentiation
response to hydrogen peroxide
response to ethanol
positive regulation of translation
response to folic acid
cell-cell adhesion
cellular response to brain-derived neurotrophic factor stimulus
positive regulation of cytoplasmic translation
translation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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