Invitrogen

Phospho-MEK1 (Thr292) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Reference
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Datasheet
Protocols
Questions & Answers

Product Details

44-458G

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200
-

Immunocytochemistry (ICC/IF)

1:250
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK1 that contains threonine 292. The sequence is conserved in many species including mouse, rat, chimp, hamster and rabbit.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533656

Target Information

MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; mitogen activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; MKK (Thr292); MKK (Thr386); MKK2; MP2K1; PRKMK2; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

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Gene Aliases: CFC3; MAP2K1; MAPKK1; MEK1; MEKK1; MKK1; PRKMK1

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UniProt ID: (Human) Q02750, (Mouse) P31938, (Rat) Q01986

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Entrez Gene ID: (Human) 5604, (Mouse) 26395, (Rat) 170851

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Function(s)
protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity MAP kinase kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity receptor signaling protein tyrosine phosphatase activity protein binding ATP binding protein C-terminus binding protein kinase binding protein serine/threonine kinase activator activity protein N-terminus binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Ras GTPase binding mitogen-activated protein kinase kinase kinase binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade activation of MAPK activity movement of cell or subcellular component chemotaxis cell cycle arrest signal transduction heart development negative regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression peptidyl-tyrosine phosphorylation cerebellar cortex formation neuron differentiation keratinocyte differentiation thyroid gland development regulation of stress-activated MAPK cascade proteolysis in other organism positive regulation of transcription, DNA-templated thymus development regulation of axon regeneration cell motility positive regulation of axonogenesis Bergmann glial cell differentiation face development trachea formation epithelial cell proliferation involved in lung morphogenesis placenta blood vessel development labyrinthine layer development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of protein serine/threonine kinase activity regulation of Golgi inheritance cellular senescence mitophagy in response to mitochondrial depolarization positive regulation of production of miRNAs involved in gene silencing by miRNA regulation of early endosome to late endosome transport regulation of protein phosphorylation regulation of vascular smooth muscle contraction protein phosphorylation response to oxidative stress mitotic nuclear division cell proliferation phosphorylation positive regulation of cell migration melanosome transport positive regulation of transcription elongation from RNA polymerase II promoter negative regulation of homotypic cell-cell adhesion peptidyl-tyrosine dephosphorylation positive regulation of GTPase activity positive regulation of cell differentiation positive regulation of Ras protein signal transduction vesicle transport along microtubule Golgi inheritance response to axon injury neuron projection morphogenesis protein heterooligomerization response to glucocorticoid lung morphogenesis intracellular signal transduction
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