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Phospho-MEK7 (Thr275) Antibody (PA5-114605) in IHC (P)
Immunohistochemistry analysis of Phospho-MEK7 (Thr275) in human ovarian cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114605) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Product Details
PA5-114605
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MKK7 around the phosphorylation site of Thr275.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899241
Product Specific Information
Antibody detects endogenous levels of MKK7 only when phosphorylated at Thr275.
Target Information
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-Jun N-terminal kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 7; JNK kinase 2; JNK-activating kinase 2; JNKK 2; MAP kinase kinase 7; MAP2K4; map2k7; map2k7 {ECO:0000312|EMBL:AAX61178.1}; MAPK/ERK kinase 7; MAPKK 4; MAPKK 7; MEK 7; mitogen activated protein kinase kinase 7; protein kinase, mitogen activated, kinase 7; SAPK kinase 4; SAPKK-1; SAPKK1; Stress-activated protein kinase kinase 4
Gene Aliases: JNKK; 2; 5930412N11Rik; JNKK 2; JNKK2; MAP2K7; MAPKK 7; MAPKK7; MEK; MEK 7; MEK7; MKK7; PRKMK7; SAPKK-4; SAPKK4; sek2; SKK4
UniProt ID: (Human) O14733, (Rat) Q4KSH7, (Mouse) Q8CE90
Entrez Gene ID: (Human) 5609, (Rat) 363855, (Mouse) 26400
magnesium ion binding
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
protein C-terminus binding
JUN kinase kinase activity
enzyme binding
protein kinase binding
protein phosphatase binding
mitogen-activated protein kinase kinase kinase binding
protein serine/threonine kinase activity
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
apoptotic process
response to osmotic stress
signal transduction
JNK cascade
activation of JUN kinase activity
response to heat
response to UV
peptidyl-tyrosine phosphorylation
positive regulation of telomere maintenance via telomerase
response to tumor necrosis factor
proteolysis in other organism
Fc-epsilon receptor signaling pathway
positive regulation of neuron apoptotic process
stress-activated MAPK cascade
positive regulation of telomerase activity
cellular response to sorbitol
positive regulation of telomere capping
positive regulation of protein phosphorylation
protein phosphorylation
MAPK cascade
response to stress
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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