Invitrogen

Phospho-PPAR alpha (Ser12) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA1-820

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:1,000
View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100-1:500
View 1 publication 1 publication

Gel Shift (GS)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325817

Product Specific Information

PA1-820 detects phospho-peroxisome proliferator activated receptor (PPAR) alpha S12 from mouse tissues.

PA1-820 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to phospho-PPAR alpha S12 from mouse adipose tissue extract.

The PA1-820 immunogen is a synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha. This peptide (Cat. # PEP-181) is available for use in neutralization and control experiments.

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: MGC2237; MGC2452; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator-activated nuclear receptor alpha variant 3; Peroxisome proliferator-activated receptor alpha; PPAR-alpha

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Gene Aliases: 4933429D07Rik; AW742785; hPPAR; NR1C1; PPAR; PPAR-alpha; PPARA; PPARalpha

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UniProt ID: (Human) Q07869, (Mouse) P23204

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Entrez Gene ID: (Human) 5465, (Mouse) 19013

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription coactivator binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding drug binding zinc ion binding lipid binding phosphatase binding protein domain specific binding ubiquitin conjugating enzyme binding protein complex binding sequence-specific DNA binding NFAT protein binding MDM2/MDM4 family protein binding receptor activity metal ion binding protein heterodimerization activity C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to hypoxia transcription initiation from RNA polymerase II promoter lipid metabolic process fatty acid metabolic process heart development epidermis development negative regulation of macrophage derived foam cell differentiation negative regulation of receptor biosynthetic process negative regulation of cholesterol storage negative regulation of sequestering of triglyceride fatty acid transport intracellular receptor signaling pathway positive regulation of fatty acid beta-oxidation negative regulation of protein binding negative regulation of appetite response to insulin circadian regulation of gene expression behavioral response to nicotine wound healing lipoprotein metabolic process regulation of circadian rhythm steroid hormone mediated signaling pathway cellular lipid metabolic process positive regulation of gluconeogenesis negative regulation of blood pressure negative regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fatty acid oxidation negative regulation of inflammatory response enamel mineralization regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter negative regulation of neuron death negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of leukocyte cell-cell adhesion negative regulation of transcription regulatory region DNA binding glucose metabolic process transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter regulation of gene expression regulation of fatty acid metabolic process rhythmic process
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