Invitrogen

Phospho-RIP1 (Ser166) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-104645

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RIPK1(Accession Q13546), corresponding to amino acid residues around phosphorylated Ser166.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816120

Product Specific Information

Antibody detects endogenous levels of RIPK1 only when phosphorylated at Ser166.

Target Information

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cell death protein RIP; FLJ39204; OTTHUMP00000015955; receptor (TNFRSF)-interacting serine-threonine kinase 1; Receptor-interacting protein 1; receptor-interacting protein kinase 1; Receptor-interacting serine/threonine-protein kinase 1; RIP-1; RIP1 (CT); serine/threonine-protein kinase RIP

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Gene Aliases: D330015H01Rik; Rinp; RIP; RIP-1; RIP1; RIPK1

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UniProt ID: (Human) Q13546, (Mouse) Q60855

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Entrez Gene ID: (Human) 8737, (Mouse) 19766

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Function(s)
protein kinase activity protein serine/threonine kinase activity death receptor binding protein binding ATP binding ubiquitin protein ligase binding protein complex binding identical protein binding death domain binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
positive regulation of protein phosphorylation apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process I-kappaB kinase/NF-kappaB signaling activation of JUN kinase activity regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death positive regulation of necrotic cell death positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production tumor necrosis factor-mediated signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway peptidyl-serine autophosphorylation positive regulation of apoptotic process positive regulation of programmed cell death positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling cellular protein catabolic process positive regulation of macrophage differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade protein autophosphorylation positive regulation of NF-kappaB transcription factor activity protein homooligomerization protein heterooligomerization positive regulation of necroptotic process T cell apoptotic process necroptotic process regulation of ATP:ADP antiporter activity cellular response to tumor necrosis factor cellular response to growth factor stimulus death-inducing signaling complex assembly apoptotic signaling pathway extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway ripoptosome assembly necroptotic signaling pathway ripoptosome assembly involved in necroptotic process regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of hydrogen peroxide-induced cell death amyloid fibril formation positive regulation of reactive oxygen species metabolic process negative regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand protein phosphorylation signal transduction positive regulation of cell death programmed cell death viral process phosphorylation positive regulation of phosphorylation positive regulation of MAPK cascade positive regulation of transferase activity regulation of reactive oxygen species metabolic process
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