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Invitrogen
Phospho-RSK1 (Thr359, Ser363) Recombinant Rabbit Monoclonal Antibody (SU03-65)
FIGURE: 1 / 6
Phospho-RSK1 (Thr359, Ser363) Antibody (MA5-32129) in ICC/IF
Immunocytochemical analysis of Phospho-RSK1 (Thr359, Ser363) in HepG2 cells using a Phospho-RSK1 (Thr359, Ser363) Monoclonal antibody (Product # MA5-32129) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32129
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SU03-65
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr359 and Ser363 of Human RSK1 p90 aa 341-390
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809420
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
RPS6KA1 (RSK1) is a growth-factor regulated serine/threonine kinase that is involved in the MAPK cascade. It is thought to control cellular proliferation and differentiation through the phosphorylation of transcription factors. RPS6KA1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPK1A; p90-RSK 1; p90RSK1; p90S6K; pp90RSK1; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RSK-1; S6 protein kinase (Rsk-1); S6K-alpha 1; S6K-alpha-1
Gene Aliases: HU-1; Mapkapk-1a; MAPKAPK1A; p90-Rsk1; p90Rsk; p90Rsk1; p90S6K; RPS6KA1; RSK; Rsk-1; RSK1; S6K-alpha-1
UniProt ID: (Human) Q15418, (Rat) Q63531, (Mouse) P18653
Entrez Gene ID: (Human) 6195, (Rat) 81771, (Mouse) 20111
magnesium ion binding
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
ribosomal protein S6 kinase activity
transcription factor binding
nucleotide binding
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein modifying enzyme
protein phosphorylation
apoptotic process
cell cycle
signal transduction
positive regulation of cell growth
intracellular signal transduction
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of translation in response to stress
regulation of DNA-templated transcription in response to stress
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
hepatocyte proliferation
positive regulation of hepatic stellate cell activation
positive regulation of transcription, DNA-templated
regulation of translation
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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