Invitrogen

Phospho-SMAD2/SMAD3 (Thr8) Polyclonal Antibody

1 Reference
View all (11) SMAD2/SMAD3 antibodies
Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-99378

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunohistochemistry (Frozen) (IHC (F))

-
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SMAD3(Accession P84022), corresponding to amino acid residues around phosphorylated Thr8.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2818311

Product Specific Information

Antibody detects endogenous levels of Smad2/3 only when phosphorylated at Threonine 8.

Target Information

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hMAD-2; hMAD-3; hSMAD3; JV15-2; JV18-1; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; Mad-related protein 2; mad3; mMad2; mMad3; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 2; mothers against DPP homolog 3; pSMAD2; Sma- and Mad-related protein 2; SMA- and MAD-related protein 3; SMAD; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3; SMAD, mothers against DPP homolog 2; SMAD, mothers against DPP homolog 3

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Gene Aliases: 7120426M23Rik; AU022421; hMAD-2; hSMAD2; HSPC193; HsT17436; JV15-2; JV18; JV18-1; LDS1C; LDS3; mad3; MADH2; MADH3; MADR2; mMad2; Smad 3; Smad-2; SMAD2; SMAD3

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UniProt ID: (Human) Q15796, (Human) P84022, (Mouse) Q62432, (Rat) O70436, (Mouse) Q8BUN5, (Rat) P84025

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Entrez Gene ID: (Human) 4087, (Human) 4088, (Mouse) 17126, (Rat) 29357, (Mouse) 17127, (Rat) 25631

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding transcription factor binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ubiquitin protein ligase binding activating transcription factor binding type I transforming growth factor beta receptor binding enhancer binding protein homodimerization activity SMAD binding metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding R-SMAD binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding RNA polymerase II activating transcription factor binding collagen binding zinc ion binding protein kinase binding chromatin DNA binding identical protein binding ubiquitin binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding beta-catenin binding enzyme binding DNA-binding transcription factor gene-specific transcriptional regulator
Process(es)
negative regulation of transcription from RNA polymerase II promoter ureteric bud development in utero embryonic development endoderm formation mesoderm formation transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly zygotic specification of dorsal/ventral axis gastrulation negative regulation of cell proliferation response to glucose post-embryonic development anterior/posterior pattern specification positive regulation of epithelial to mesenchymal transition regulation of transforming growth factor beta receptor signaling pathway signal transduction involved in regulation of gene expression insulin secretion lung development negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway pancreas development primary miRNA processing activin receptor signaling pathway somatic stem cell population maintenance organ growth intracellular signal transduction nodal signaling pathway cell fate commitment negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of binding palate development pericardium development SMAD protein signal transduction response to cholesterol positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry response to hypoxia somitogenesis liver development heart looping osteoblast development immune system development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transport activation of cysteine-type endopeptidase activity involved in apoptotic process immune response cell cycle arrest endoderm development embryonic pattern specification positive regulation of gene expression positive regulation of alkaline phosphatase activity regulation of striated muscle tissue development evasion or tolerance of host defenses by virus negative regulation of cell growth positive regulation of cell migration positive regulation of bone mineralization thyroid gland development positive regulation of chondrocyte differentiation positive regulation of interleukin-1 beta production regulation of transforming growth factor beta2 production positive regulation of transforming growth factor beta3 production negative regulation of osteoblast proliferation wound healing T cell activation positive regulation of transcription factor import into nucleus negative regulation of apoptotic process cell-cell junction organization negative regulation of fat cell differentiation negative regulation of osteoblast differentiation negative regulation of mitotic cell cycle developmental growth regulation of epithelial cell proliferation negative regulation of inflammatory response regulation of immune response positive regulation of positive chemotaxis positive regulation of stress fiber assembly positive regulation of focal adhesion assembly transdifferentiation negative regulation of wound healing lens fiber cell differentiation extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway positive regulation of extracellular matrix assembly regulation of transcription, DNA-templated protein phosphorylation pattern specification process heart development negative regulation of gene expression skeletal system development osteoblast differentiation negative regulation of protein phosphorylation positive regulation of catenin import into nucleus negative regulation of protein catabolic process protein stabilization positive regulation of canonical Wnt signaling pathway
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