Proteintech

Phospho-TDP43 (Ser403, Ser404) Monoclonal Antibody (6B11B12)

Phospho-TDP43 (Ser403, Ser404) Antibody in Western Blot (WB)

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Phospho-TDP43 (Ser403, Ser404) Antibody (66079-1-IG) in WB

Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66079-1-Ig (phospho (403/404)-TDP43 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Phospho-TDP43 (Ser403, Ser404) Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

66079-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Zebrafish

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6B11B12

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.14 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Product # 66079-1-Ig is a mouse monoclonal antibody recognizing TDP-43 only when phosphorylated at 403/404. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Target Information

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells.

Methods in molecular biology (Clifton, N.J.)

Gruijs da Silva LA,Dormann D

Mon Oct 17 00:00:00 EDT 2022

PIKFYVE inhibition mitigates disease in models of diverse forms of ALS.

Cell

Hung ST,Linares GR,Chang WH,Eoh Y,Krishnan G,Mendonca S,Hong S,Shi Y,Santana M,Kueth C,Macklin-Isquierdo S,Perry S,Duhaime S,Maios C,Chang J,Perez J,Couto A,Lai J,Li Y,Alworth SV,Hendricks E,Wang Y,Zlokovic BV,Dickman DK,Parker JA,Zarnescu DC,Gao FB,Ichida JK

Thu Feb 16 00:00:00 EST 2023

TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury.

Nature neuroscience

Zambusi A,Novoselc KT,Hutten S,Kalpazidou S,Koupourtidou C,Schieweck R,Aschenbroich S,Silva L,Yazgili AS,van Bebber F,Schmid B,Möller G,Tritscher C,Stigloher C,Delbridge C,Sirko S,Günes ZI,Liebscher S,Schlegel J,Aliee H,Theis F,Meiners S,Kiebler M,Dormann D,Ninkovic J

Thu Dec 01 00:00:00 EST 2022

TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury.

Nature neuroscience

Thu Dec 01 00:00:00 EST 2022

TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury.

Nature neuroscience

Thu Dec 01 00:00:00 EST 2022

TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury.

Nature neuroscience

Thu Dec 01 00:00:00 EST 2022

Targeting nuclear protein TDP-43 by cell division cycle kinase 7 inhibitors: A new therapeutic approach for amyotrophic lateral sclerosis.

European journal of medicinal chemistry

Rojas-Prats E,Martinez-Gonzalez L,Gonzalo-Consuegra C,Liachko NF,Perez C,Ramírez D,Kraemer BC,Martin-Requero Á,Perez DI,Gil C,de Lago E,Martinez A

Fri Jan 15 00:00:00 EST 2021

Influence of APOE genotype in primary age-related tauopathy.

Acta neuropathologica communications

Robinson AC,Davidson YS,Roncaroli F,Minshull J,Tinkler P,Horan MA,Payton A,Pendleton N,Mann DMA

Mon Dec 07 00:00:00 EST 2020

Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.

Biochemistry

Ng JSW,Hanspal MA,Matharu NS,Barros TP,Esbjörner EK,Wilson MR,Yerbury JJ,Dobson CM,Kumita JR

Tue Oct 01 00:00:00 EDT 2019

Drosophila lines with mutant and wild type human TDP-43 replacing the endogenous gene reveals phosphorylation and ubiquitination in mutant lines in the absence of viability or lifespan defects.

PloS one

Chang JC,Morton DB

Fri Sep 22 00:00:00 EDT 2017

Drosophila lines with mutant and wild type human TDP-43 replacing the endogenous gene reveals phosphorylation and ubiquitination in mutant lines in the absence of viability or lifespan defects.

PloS one

Chang JC,Morton DB

Fri Sep 22 00:00:00 EDT 2017

Clinicopathological description of two cases with SQSTM1 gene mutation associated with frontotemporal dementia.

Neuropathology : official journal of the Japanese Society of Neuropathology

Kovacs GG,van der Zee J,Hort J,Kristoferitsch W,Leitha T,Höftberger R,Ströbel T,Van Broeckhoven C,Matej R

Mon Feb 01 00:00:00 EST 2016

Bioinformatics

Protein Aliases: OTTHUMP00000002171; OTTHUMP00000002173; RP4-635E18.2; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TDP-43

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Gene Aliases: 1190002A23Rik; ALS10; C85084; TARDBP; TDP-43; TDP43

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UniProt ID: (Human) Q13148, (Mouse) Q921F2

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Entrez Gene ID: (Human) 23435, (Mouse) 230908

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Function(s)

nucleotide binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA binding mRNA 3'-UTR binding protein binding identical protein binding poly(A) RNA binding nucleic acid binding DNA binding

Process(es)

negative regulation of protein phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mRNA processing RNA splicing negative regulation of gene expression nuclear fragmentation involved in apoptotic nuclear change negative regulation by host of viral transcription positive regulation of transcription from RNA polymerase II promoter regulation of cell cycle 3'-UTR-mediated mRNA stabilization nuclear inner membrane organization positive regulation of insulin secretion response to endoplasmic reticulum stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-TDP43 (Ser403, Ser404) Monoclonal Antibody (6B11B12)

Phospho-TDP43 (Ser403, Ser404) Antibody (66079-1-IG) in WB

Product Details

66079-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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