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FIGURE: 1 / 1
Phospho-Talin (Ser425) Antibody (PA5-143776) in WB
Western blot of Phospho-Talin (Ser425) in rat PC12 cells stimulated with Calyculin A (100 nM) for 30 minutes (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit Phospho-Talin (Ser425) polyclonal antibody (Product # PA5-143776) (lanes 3 & 4).
Product Details
PA5-143776
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-Talin (Ser-425) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-425 in human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2 (Ser-428).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2943004
Product Specific Information
The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.
Target Information
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: talin 2; Talin-1; Talin-2
Gene Aliases: 5730421P04Rik; AI507121; AI787438; AL118320; ILWEQ; KIAA0320; KIAA1027; mKIAA0320; RGD1565416; TLN; TLN1; TLN2
UniProt ID: (Human) Q9Y490, (Human) Q9Y4G6, (Mouse) P26039, (Mouse) Q71LX4
Entrez Gene ID: (Human) 7094, (Human) 83660, (Rat) 313494, (Mouse) 21894, (Mouse) 70549, (Rat) 315776
integrin binding
structural constituent of cytoskeleton
protein binding
vinculin binding
LIM domain binding
actin filament binding
cadherin binding involved in cell-cell adhesion
actin binding
structural molecule activity
molecular_function
insulin receptor binding
protein complex binding
non-motor actin binding protein
platelet degranulation
movement of cell or subcellular component
muscle contraction
cytoskeletal anchoring at plasma membrane
cell-cell junction assembly
cell-substrate junction assembly
viral process
cortical actin cytoskeleton organization
IRE1-mediated unfolded protein response
platelet aggregation
cell adhesion
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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