Invitrogen

Phospho-Tau (Thr217) Polyclonal Antibody

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Tech Support

Antibody Testing Data (3)
Published Figures (7)

Phospho-Tau (Thr217) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-Tau (Thr217) Antibody (44-744) in IHC (P)

Immunohistochemistry analysis of Phospho-Tau (pT217) showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room te... View More

Phospho-Tau (Thr217) Antibody in Western Blot (WB)

Phospho-Tau (Thr217) Antibody in Immunohistochemistry (IHC)

Phospho-Tau (Thr217) Antibody in ELISA (ELISA)

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Product Details

44-744

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

View 1 publication 1 publication

ELISA (ELISA)

View 3 publications 3 publications

Functional Assay (Functional)

View 1 publication 1 publication

Dot blot (DB)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Baboon, Bovine, Dog, Human, Mouse, Non-human primate, Rat, Sheep, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human tau that contains threonine 217.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533741

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Drosophila Toll-9 is induced by aging and neurodegeneration to modulate stress signaling and its deficiency exacerbates tau-mediated neurodegeneration.

iScience

Sakakibara Y,Yamashiro R,Chikamatsu S,Hirota Y,Tsubokawa Y,Nishijima R,Takei K,Sekiya M,Iijima KM

Published figure using Phospho-Tau (Thr217) polyclonal antibody (Product # 44-744) in Western Blot

Fri Feb 17 00:00:00 EST 2023

Organotypic Hippocampal Slice Cultures from Adult Tauopathy Mice and Theragnostic Evaluation of Nanomaterial Phospho-TAU Antibody-Conjugates.

Cells

Kemppainen S,Huber N,Willman RM,Zamora A,Mäkinen P,Martiskainen H,Takalo M,Haapasalo A,Sobrino T,González Gómez MA,Piñeiro Y,Rivas J,Himmelreich U,Hiltunen M

44-744 was used in Immunohistochemistry to establish an adult-originating slice culture system for tauopathy studies.

Thu May 18 00:00:00 EDT 2023

Abundance of Nef and p-Tau217 in Brains of Individuals Diagnosed with HIV-Associated Neurocognitive Disorders Correlate with Disease Severance.

Molecular neurobiology

Pushkarsky T,Ward A,Ivanov A,Lin X,Sviridov D,Nekhai S,Bukrinsky MI

44-744 was used in Simple Western - Size to highlight the contribution of Nef and Nef-dependent impairment of cholesterol efflux to HAND pathogenesis and support a connection between the pathogenesis of HAND and Alzheimer's disease.

Tue Feb 01 00:00:00 EST 2022

Long-Term Depression-Inducing Low Frequency Stimulation Enhances p-Tau181 and p-Tau217 in an Age-Dependent Manner in Live Rats.

Journal of Alzheimer's disease : JAD

Zhang Y,Yang Y,Hu Z,Zhu M,Qin S,Yu P,Li B,Xu J,Ondrejcak T,Klyubin I,Rowan MJ,Hu NW

44-744 was used in Immunohistochemistry-immunofluorescence to provide a novel in vivo means to uncover brain plasticity-related cellular and molecular processes of tau phosphorylation at key sites in health and aging.

Thu Sep 08 00:00:00 EDT 2022

Amelioration of Alzheimer's disease pathology by mitophagy inducers identified via machine learning and a cross-species workflow.

Nature biomedical engineering

Xie C,Zhuang XX,Niu Z,Ai R,Lautrup S,Zheng S,Jiang Y,Han R,Gupta TS,Cao S,Lagartos-Donate MJ,Cai CZ,Xie LM,Caponio D,Wang WW,Schmauck-Medina T,Zhang J,Wang HL,Lou G,Xiao X,Zheng W,Palikaras K,Yang G,Caldwell KA,Caldwell GA,Shen HM,Nilsen H,Lu JH,Fang EF

44-744 was used in Western Blot to report the combined use of unsupervised machine learning and a cross-species approach for the screening and experimental validation of new mitophagy-inducing compounds.

Sat Jan 01 00:00:00 EST 2022

Amelioration of Alzheimer's disease pathology by mitophagy inducers identified via machine learning and a cross-species workflow.

Nature biomedical engineering

Sat Jan 01 00:00:00 EST 2022

Differential expression of tau species and the association with cognitive decline and synaptic loss in Alzheimer's disease.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Saroja SR,Sharma A,Hof PR,Pereira AC

44-744 was used in Western Blotting to propose that tau species from NFT-predominant tissues possess greater levels of degenerative properties, thereby causing synaptic loss and cognitive decline.

Thu Sep 01 00:00:00 EDT 2022

Cerebrospinal fluid p-tau231 as an early indicator of emerging pathology in Alzheimer's disease.

EBioMedicine

Ashton NJ,Benedet AL,Pascoal TA,Karikari TK,Lantero-Rodriguez J,Brum WS,Mathotaarachchi S,Therriault J,Savard M,Chamoun M,Stoops E,Francois C,Vanmechelen E,Gauthier S,Zimmer ER,Zetterberg H,Blennow K,Rosa-Neto P

44-744 was used in Functional assay to demonstrate that CSF p-tau231 increases early in development of AD pathology and is a principal candidate for detecting incipient Aβ pathology for therapeutic trial application.

Tue Feb 01 00:00:00 EST 2022

Head-to-head comparison of clinical performance of CSF phospho-tau T181 and T217 biomarkers for Alzheimer's disease diagnosis.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Karikari TK,Emeršič A,Vrillon A,Lantero-Rodriguez J,Ashton NJ,Kramberger MG,Dumurgier J,Hourregue C,Čučnik S,Brinkmalm G,Rot U,Zetterberg H,Paquet C,Blennow K

44-744 was used in ELISA to performed a head-to-head comparison of different p-tau biomarker performances for Alzheimer's disease.

Sat May 01 00:00:00 EDT 2021

Dephosphorylation Passivates the Seeding Activity of Oligomeric Tau Derived From Alzheimer's Brain.

Frontiers in molecular neuroscience

Wu R,Li L,Shi R,Zhou Y,Jin N,Gu J,Tung YC,Liu F,Chu D

Published figure using Phospho-Tau (Thr217) polyclonal antibody (Product # 44-744) in Western Blot

Tue Jun 01 00:00:00 EDT 2021

Systematic Phenotyping and Characterization of the 3xTg-AD Mouse Model of Alzheimer's Disease.

Frontiers in neuroscience

Javonillo DI,Tran KM,Phan J,Hingco E,Kramár EA,da Cunha C,Forner S,Kawauchi S,Milinkeviciute G,Gomez-Arboledas A,Neumann J,Banh CE,Huynh M,Matheos DP,Rezaie N,Alcantara JA,Mortazavi A,Wood MA,Tenner AJ,MacGregor GR,Green KN,LaFerla FM

Published figure using Phospho-Tau (Thr217) polyclonal antibody (Product # 44-744) in Immunohistochemistry

Sat Dec 17 00:00:00 EST 2022

Systematic Phenotyping and Characterization of the 3xTg-AD Mouse Model of Alzheimer's Disease.

Frontiers in neuroscience

Published figure using Phospho-Tau (Thr217) polyclonal antibody (Product # 44-744) in Immunohistochemistry

Sat Dec 17 00:00:00 EST 2022

Novel tau biomarkers phosphorylated at T181, T217 or T231 rise in the initial stages of the preclinical Alzheimer's continuum when only subtle changes in Aβ pathology are detected.

EMBO molecular medicine

Suárez-Calvet M,Karikari TK,Ashton NJ,Lantero Rodríguez J,Milà-Alomà M,Gispert JD,Salvadó G,Minguillon C,Fauria K,Shekari M,Grau-Rivera O,Arenaza-Urquijo EM,Sala-Vila A,Sánchez-Benavides G,González-de-Echávarri JM,Kollmorgen G,Stoops E,Vanmechelen E,Zetterberg H,Blennow K,Molinuevo JL

44-744 was used in Antibody array to support the idea that early changes in neuronal tau metabolism in preclinical Alzheimer, likely in response to Aβ exposure, can be detected with these novel p-tau assays.

Mon Dec 07 00:00:00 EST 2020

Icariin protects against sodium azide-induced neurotoxicity by activating the PI3K/Akt/GSK-3β signaling pathway.

PeerJ

Zhang Y,Huang N,Lu H,Huang J,Jin H,Shi J,Jin F

Published figure using Phospho-Tau (Thr217) polyclonal antibody (Product # 44-744) in Western Blot

Thu Apr 14 00:00:00 EDT 2022

Truncation of Tau selectively facilitates its pathological activities.

The Journal of biological chemistry

Gu J,Xu W,Jin N,Li L,Zhou Y,Chu D,Gong CX,Iqbal K,Liu F

44-744 was used in Western Blotting to suggest that the first 150 aa and the last 50 aa protect Tau from pathological characteristics and that their deletions facilitate pathological activities.

Fri Oct 02 00:00:00 EDT 2020

Pathological Tau From Alzheimer's Brain Induces Site-Specific Hyperphosphorylation and SDS- and Reducing Agent-Resistant Aggregation of Tau in vivo.

Frontiers in aging neuroscience

Miao J,Shi R,Li L,Chen F,Zhou Y,Tung YC,Hu W,Gong CX,Iqbal K,Liu F

44-744 was used in Western Blotting to speculate that AD P-tau seeds hyperphosphorylated tau to form aggregates, which resist to the dephosphorylation by PP2A, resulting in hyperphosphorylation and pathology of tau.

Thu Oct 01 00:00:00 EDT 2020

A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer's disease brain.

Acta neuropathologica communications

Ercan-Herbst E,Ehrig J,Schöndorf DC,Behrendt A,Klaus B,Gomez Ramos B,Prat Oriol N,Weber C,Ehrnhoefer DE

Published figure using Phospho-Tau (Thr217) polyclonal antibody (Product # 44-744) in ELISA

Tue Dec 03 00:00:00 EST 2019

Relevance of Phosphorylation and Truncation of Tau to the Etiopathogenesis of Alzheimer's Disease.

Frontiers in aging neuroscience

Zhou Y,Shi J,Chu D,Hu W,Guan Z,Gong CX,Iqbal K,Liu F

Published figure using Phospho-Tau (Thr217) polyclonal antibody (Product # 44-744) in Western Blot

Sat Apr 09 00:00:00 EDT 2022

Expression of Tau Pathology-Related Proteins in Different Brain Regions: A Molecular Basis of Tau Pathogenesis.

Frontiers in aging neuroscience

Hu W,Wu F,Zhang Y,Gong CX,Iqbal K,Liu F

44-744 was used in Dot blot to provide the regional expression profiles of tau and tau pathology-related proteins in the brain.

Thu Oct 01 00:00:00 EDT 2020

Liraglutide Improves Water Maze Learning and Memory Performance While Reduces Hyperphosphorylation of Tau and Neurofilaments in APP/PS1/Tau Triple Transgenic Mice.

Neurochemical research

Chen S,Sun J,Zhao G,Guo A,Chen Y,Fu R,Deng Y

44744 was used in western blot to elucidate how liraglutide affects disease and cognitive function in a mouse model of Alzheimer disease

Tue Aug 01 00:00:00 EDT 2017

Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment.

The Journal of comparative neurology

Smolek T,Madari A,Farbakova J,Kandrac O,Jadhav S,Cente M,Brezovakova V,Novak M,Zilka N