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Phospho-VAV2 (Tyr142) Antibody (PA5-143787) in ICC/IF
Immunocytochemistry analysis of Phospho-VAV2 (Tyr142) in control and pervanadate-treated human A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40. Then labeled with rabbit Phospho-VAV2 (Tyr142) polyclonal antibody (Product # PA5-143787). The antibody was detected using goat anti-rabbit DyLight® 594.
Product Details
PA5-143787
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-Vav2 (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-142 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2943015
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated Vav2 (Tyr-142) peptide before affinity purification using phospho-Vav2 (Tyr-142) peptide (without carrier). The antibody detects a 100 kDa* band corresponding to Vav on SDS-PAGE immunoblots of human A431 cells treated with EGF and detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.
Target Information
VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Guanine nucleotide exchange factor VAV2; Oncogene VAV2; vav 2 guanine nucleotide exchange factor; vav 2 oncogene; VAV-2; Vav2 oncogene
Gene Aliases: 2810040F13Rik; AI847175; VAV-2; VAV2
UniProt ID: (Human) P52735, (Mouse) Q60992
Entrez Gene ID: (Human) 7410, (Rat) 296603, (Mouse) 22325
angiogenesis
signal transduction
small GTPase mediated signal transduction
regulation of cell size
regulation of gene expression
cell migration
lamellipodium assembly
platelet activation
regulation of blood coagulation
regulation of Rho protein signal transduction
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
positive regulation of apoptotic process
regulation of GTPase activity
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
regulation of small GTPase mediated signal transduction
cell projection assembly
intracellular signal transduction
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