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Phospho-WNK4 (Ser575) Antibody (PA5-114673) in IHC (P)
Immunohistochemistry analysis of Phospho-WNK4 (Ser575) in mouse stomach tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114673) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Product Details
PA5-114673
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human WNK4 around the phosphorylation site of Ser575.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899309
Product Specific Information
Antibody detects endogenous levels of WNK4 only when phosphorylated at Ser575.
Target Information
This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein kinase lysine-deficient 4; Protein kinase with no lysine 4; protein kinase, lysine deficient 4; protein kinase, lysine-deficient 4; RP23-281C18.8; Serine/threonine-protein kinase WNK4; WNK4 Ser/Thr kinase
Gene Aliases: 2010002J11Rik; Ac2-059; PHA2B; PRKWNK4; WNK4
UniProt ID: (Human) Q96J92, (Mouse) Q80UE6, (Rat) Q7TPK6
Entrez Gene ID: (Human) 65266, (Mouse) 69847, (Rat) 287715
protein phosphorylation
ion transport
chloride transport
protein localization
positive regulation of ion transmembrane transporter activity
intracellular signal transduction
regulation of cellular process
ion homeostasis
renal sodium ion absorption
distal tubule morphogenesis
negative regulation of pancreatic juice secretion
regulation of ion homeostasis
phosphorylation
cellular chloride ion homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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