Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
Phospho-YAP1 (Ser128) Antibody (PA5-117264) in WB
Western blot analysis of Phospho-YAP1 (Ser128) in extracts of NIH/3T3 cells. Samples were incubated with Phospho-YAP1 (Ser128) Polyclonal antibody (Product # PA5-117264) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by Forskolin (30 µM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins:... View More
Product Details
PA5-117264
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S128 of human YAP1 (NP_0011236171)
Conjugate
Form
Liquid
Concentration
2.1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901894
Product Specific Information
Positive Samples: NIH/3T3
Immunogen sequence: HSSPA
Target Information
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; Yes-associated protein 1; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog
Gene Aliases: AI325207; Yap; Yap1; Yap65; Yki; Yorkie
UniProt ID: (Mouse) P46938
Entrez Gene ID: (Mouse) 22601
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
chromatin binding
transcription coactivator activity
transcription corepressor activity
protein binding
protein C-terminus binding
transcription regulatory region DNA binding
proline-rich region binding
transcription cofactor
cell morphogenesis
vasculogenesis
embryonic heart tube morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
cell proliferation
positive regulation of cell proliferation
regulation of keratinocyte proliferation
keratinocyte differentiation
negative regulation of epithelial cell differentiation
notochord development
somatic stem cell population maintenance
hippo signaling
regulation of cell proliferation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of organ growth
paraxial mesoderm development
lateral mesoderm development
regulation of neurogenesis
contact inhibition
bud elongation involved in lung branching
lung epithelial cell differentiation
regulation of canonical Wnt signaling pathway
cellular response to retinoic acid
cellular response to gamma radiation
regulation of stem cell proliferation
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of canonical Wnt signaling pathway
positive regulation of stem cell population maintenance
negative regulation of nucleic acid-templated transcription
negative regulation of stem cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support